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XB-ANTIBODY-16331798

Antibody Name: Hnf1a Ab1

Common Name: XAD5

RRID:
Synonyms: XAD5, XLFB1, XLFB1a, XLFB1b

Antigens
Gene: hnf1a
XAO: alimentary system, liver, gall bladder, epithelium

Clone Type: Monoclonal
Source: Ryffel Lab

Antibody
Clone Number XAD5
Purification
Isotype isotype unknown
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity
Description: Generated by Ryffel lab in 1993
Immunogen
Name Hnf1a
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description The 3'-terminal EcoRI fragment of the al cDNA clone, encoding the activation domain of XLFBla spanning from the EcoRI site at position 1058 (see Fig. 1) to the EcoRI site in the cloning adaptor, was ligated into the EcoRI site of prokaryotic expression vector pGEX-3X (Pharmacia). The fusion protein was purified from isopropyl-o-D-thiogalactopyranoside (IPTG)-induced bacteria on a glutathione-Sepharose 4B column (Pharmacia) as described by the manufacturer. AA 1-605
Sequence
Reported Usage
immunohistochemistry
western blot
Publications
First: Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis., Mol Cell Biol 1993  
Most recent: Mesoderm and endoderm differentiation in animal cap explants: identification of the HNF4-binding site as an activin A responsive element in the Xenopus HNF1alpha promoter., Development 1996                
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