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XB-ANTIBODY-18258718

Antibody Name: Tcof1 Ab1

Common Name: treacle

RRID:
Synonyms: xtreacle

Antigens
Gene: tcof1

Clone Type: unknown
Source:

Antibody
Clone Number
Purification
Isotype IgG
Host Organism chicken
Xenopus Reactivity Tropicalis, Laevis
Non-Xenopus Reactivity
Description: The 5′ end of xTcof1 cDNA was amplified by PCR using BV1180 (5′-ACTCAGATCCCATGGAGGAAGTGGAGGGATCC-3′) and BV1181 (5′-TCCAGAGCTCTCGAGTCACTGTGCAATCACAGGTGT-3′) and subcloned into the NcoI and XhoI sites of pPROEX HTa vector. The E. coli-expressed poly-histidine-tagged protein, which included amino acids 1–307 of Xenopus treacle (xtreacle), was purified using Ni-NTA agarose (Qiagen, Inc.) and sent to Sigma for antibody production in chicken.
Immunogen
Name tcof1
Type polypeptide
Post Translational Modifications None
Source Organism chicken
Description
Sequence
Reported Usage
immunofluorescence
western blot
Publications
First: Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene product. [Gene 2005]
Most recent:
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