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XB-ANTIBODY-18258802

Antibody Name: Rho Ab10

Common Name: Rhodopsin

RRID: AB_477464
Synonyms: 1D4, R5403, Rhodopsin 1D4

Antigens
XAO: retina, photoreceptor layer, retinal cone cell

Clone Type: Monoclonal
Source: Sigma-Aldrich

Antibody
Clone Number 1D4
Purification Affinity Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity human, rat
Description: Sigma-Aldrich Cat# R5403. May only bind artificial constructs of mammalian rhodopsin, pay particular attention to details of experiments using this antibody
Immunogen
Name rho
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description Fimmuogen is a bovine rhodopsin. By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
Sequence
Reported Usage
immunohistochemistry
Publications
First: Noggin 1 overexpression in retinal progenitors affects bipolar cell generation., Int J Dev Biol 2016          
Most recent: Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa., J Neurosci 2017                    
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