| Summary |
|
| Synonym: |
hsp70:dnYap-2A-GFP, gCry:tdTomato |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Outbred
|
| Paternal Line: |
|
| Maternal Line: |
|
| Description: |
330bp hsp70 promoter driving dominant negative form of Yes associated protein 1 (yap1) tagged with GFP via 2A peptides; and 2.2 kb gamma crystalline promoter (cryga) driving tdTomato. DNyap1 (labeled with GFP) specifically blocks Yap1 function in Hippo signaling and dnYap-2A-GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block yap1 function temporally at desired time-point by a heat-shock. |
| Phenotype Description: |
DNyap1-2A-GFP can be induced by a heat-shock; tdTomato (red) fluorescence in the lens. Stage-dependent phenotypes related to disrupted HIPPO signaling pathway. |
| Color Morph: |
|
| Breeding Type: |
UNKNOWN
|
| Lab of Origin: |
Tamura Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
|
| MTA Required: |
No
|
| Public: |
Yes
|
| Catalogue Number: |
EXRC line #85 |
