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Summary Attributions Transgene
XB-LINE-803

Line Name: Xla.Tg(Xtr.ins:DNthra-eGFP)1Brown


Summary
Synonym: pcs2::Insulin::TRDN-GFP3
Species: Xenopus laevis
Background Strain: Unknown
Paternal Line:
Maternal Line:
Color Morph: unknown
Phenotype Description: GFP in pancreas Beta-cells.
Breeding Type: UNKNOWN
Description: 5.4 kb X. tropicalis insulin promoter driving dominant negative form of the thyroid receptor alpha ( (aka TRDN)) fused to GFP in beta-cells. The pInsulin-TRDN-GFPconstruct was made by amplifying the 5.4 kb fragment of the promoter by PCR using primers that incorporated a Xho1 site at the 5' end and Sma1 at the 3' end, and this PCR-product was ligated into the pCS2-CMV-TRDN-flex-GFP that had been cleaved by Sal1 (at 5'and HindIII (at 3') replacing the existing CMV promoter sequence.
Lab of Origin: Brown Lab
Line Type: Transgenic
Mutated Gene:
MTA Required: Yes
Public: Yes
Catalogue Number:

Vendor RRID Availability
NXR (US): No
EXRC (Europe): No
NBRP (Japan): No
XLRRI (US): No
CRB (Europe): No

     

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