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Summary Attributions Transgene
XB-LINE-920

Line Name: Xla.Tg(kdr:eGFP)Pec


Summary
Synonym: Xl.Tg(flk1:eGFP)
Species: Xenopus laevis
Background Strain: Unknown
Colour Morph: albino
Phenotype Description: GFP expression in blood and lymphatic vessels. Earliest specific GFP signal visible is at NF stage 35-36 (in posterior cardinal vein and intersomitic vessels). GFP signal in all blood vessels and lymphatic vessels from NF stage 42 onwards.
Breeding Type: UNKNOWN
Description: The Xenopus laevis Flk1 (VEGFR-2) promoter and first intron were used to drive GFP. See Figure 1 and Figure 2 in Ny et. Al. Biol Open. 2013 Jul 11; 2(9):882-90. A genomic fragment containing 2.5 kb of promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements are essential for the correct expression pattern, was isolated and cloned into pBSK(+) Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the xlFlk1 promoter. REMI method.
Lab of Origin: Carmeliet Lab
Line Type: Transgenic
MTA Required: No
Public: Yes
RRID:
Catalogue number: waiting for EXRC RRID
Vendor Availability:
     NXR (US) No
     EXRC (Europe) Yes, order
     NBRP (Japan) No

     

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