Description: |
This inducer line contains the chondrocyte-specific regulatory elements from the 5' region and first intron of the Xenopus tropicalis col2a1 gene (Kerney et al. 2010). These activate the tetracycline/doxycycline conditional transcription factor rtTA, which in the presence of doxycycline (Dox), induces expression of Cre-recombinase (Cre) via the tetracycline/
doxycycline response element (TRE) promoter in our transgenic line. The spatial expression of rtTA is restricted to cartilage by col2a1 regulatory elements. Temporal control of TRE activation by rtTA depends on the addition of Dox to the rearing medium.
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