|
XB-TRANSGENE-19756062
| Transgene Name | Tg(Xtr.hal2:eGFP,cryga:GFP3) |
| Description | X. tropicalis HAL2 promoter region was PCR amplified- the product was used for the nested PCR with primers bearing a KpnI site and an AgeI site, digested with KpnI and AgeI, and cloned into AcGFP1.1 vector (Clontech). A 4.4-kb HAL2 promoter in AcGFP1.1 was subcloned into the KpnI-AgeI digested double-promoter construct pDPCG-SceI -to produce a transgenic construct with the HAL2 promoter driving eGFP expression and cryga in lens,which allows easy identification of transgenic tadpoles |
| Inducibility | None |
| Components |
Return to Transgene list |