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XB-TRANSGENE-22251778
| Transgene Name | Tg(six1-GR) |
| Description | To generate a hormone-inducible Six1expression vector (pCS2+-GR-myc-Six1), the full open-reading frame of Xenopus Six1 was generated by PCR with primers containing XhoI sites and the pDH105-Six1 plasmid (Brugmann et al., 2004). The Six1fragment was inserted into the XhoI site downstream of GR-myc in pCS2+-GR-myc. Both plasmids were confirmed by sequencing and used as templates to generate mRNAs (GR–Six1). |
| Inducibility | None |
| Components |
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