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Fig. 4. Utilization of hnRNP E2 mRNA in oocyte and embryos. (A) Cytoplasmic
extracts from oocytes and embryos of different stages fractionated
on sucrose gradients. Poly(A) RNA was extracted from polysome and
subpolysomal fractions and analyzed by Northern blot hybridization with
the hnRNP E2 probe. The RNA loaded on each gel corresponds to the same
oocyte and embryo number of individuals. As a control, the same filter was
hybridized to a probe for histone H3. Stages are indicated at left. (B)
Analysis of hnRNP E2 in oocytes and embryos by Western blot. A total
of 90 mg of protein from cytoplasmic soluble extracts were loaded on
protein gels, blotted and incubated with an antiserum versus X. laevis
hnRNP E2. The filter was also incubated with an antiserum developed
versus Xenopus recombinant La. Numbers refer to developmental stages. |
