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Figure 9. Shroom proteins control cell heightening. A–C: Ectopic Shroom2 induces cell heightening in naive epithelial cells. A: Control. B: Shroom2-expressing cell. Green, γ-tubulin; red, myc-Shroom2). C: Graph of apicobasal cell height of control and Shroom2-expressing cells (mean ± s.e.m.; ctl, n = 16; Shroom2, n = 15). D–H: Ectopic Shroom2 expression induces cell heightening in epidermal cells. D: Control. E: Shroom2-expressing epidermis. F: Shroom3-expressing epidermis. Green, α-tubulin; red, myc-tagged Shroom2 or 3. G: Graph of apicobasal cell height in Shroom2-expressing epidermal cells (mean ± s.e.m.; ctl, n = 25;Shroom2, n = 25). H: Graph of apicobasal cell height in Shroom3-expressing epidermal cells (mean ± s.e.m : ctl, n = 39;Shroom3, n = 40). Scale bar = 20 μm. Yellow arrow bars are showing the way to measure the cell height for control cells (neighboring cells) and pink arrow bars are showing that for Shroom-expressing cells. I,L: Loss of function assay for Shroom2 in neural plate. Transverse cross-section view of neural plate. Green, α-tubulin; red, propidium iodide. I: One side-Shroom2 mismatched morpholino (MM)-injected embryo. J: One side-Shroom2 morpholino (MO)-injected embryo. The high-magnification views of control side cells (K) and Shroom2 MO-injected side cells (L). Scale bar = 50 μm. |
