Return to search results Title: RISC factor MOV10 is required for normal development and viability in both mouse and Xenopus

Summary

MOV10 is an RNA helicase that functions in the miRNA pathway. Our study shows that a knockdown of MOV10 in mouse is embryonic lethal. To determine the cause of this developmental phenotype, we knocked down maternal MOV10 in Xenopus eggs at the one cell stage. We observe a defective Maternal to Zygotic Transition (MZT) phenotype in the MOV10 knockouts that leads to abnormal neural tube formation. We are carrying out this RNA seq in order to ascertain the mRNA changes caused on maternal MOV10 knockdown

Contributors: Stephanie Ceman, Geena Skariah

Experiment Type: The analysis includes 5 samples. Three of these are control morpholino injected stage 10.5 embryos. The remaining 2 are MOV10 morpholino treated embryos from the same stage. The samples are biological replicates.

Experiment Reagents: mov10 MO1.


Article: XB-ART-54381, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
WE - NF10.5 GSM2301411  GSM2301412  GSM2301413  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection   NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5      WE + mov10 MO - NF10.5 DEG GSM2301414  GSM2301415  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection mov10 MO1 mov10 (Xla) NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5

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