Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
3'-end-dependent formation of U6 small nuclear ribonucleoprotein particles in Xenopus laevis oocyte nuclei.
Terns MP
,
Lund E
,
Dahlberg JE
.
???displayArticle.abstract???
We have identified and characterized a U6 small nuclear (sn) ribonucleoprotein particle (RNP) present in the nuclei of Xenopus laevis oocytes. The structure of this U6 snRNP was investigated by native gel shift analysis and a combination of RNA-protein UV cross-linking, RNase T1 fingerprinting, and immunoprecipitation assays. These analyses demonstrate that certain forms of U6 snRNA associate with the 50-kDa nuclear antigen La both in vivo and in vitro. The La protein binds the stretch of uridylates at the 3' hydroxyl end of newly synthesized U6 snRNA. La does not bind to mature U6 snRNAs that have 2',3'-cyclic phosphate (greater than p) groups at their 3' ends (E. Lund and J. E. Dahlberg, Science 255:327-330, 1992) or to U6 snRNAs in anti-Sm-precipitable U4/U6 snRNPs. We propose that 3'-end modification, including posttranscriptional UMP addition, modulates the binding of La protein to U6 snRNA which, in turn, may affect the function of this RNA.
Behrens,
Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.
1991, Pubmed
Behrens,
Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.
1991,
Pubmed
Black,
U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.
1989,
Pubmed
Bringmann,
Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.
1984,
Pubmed
Brow,
Spliceosomal RNA U6 is remarkably conserved from yeast to mammals.
1988,
Pubmed
Brow,
Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop.
1987,
Pubmed
Brow,
In vitro transcripts of a yeast variant 5 S rRNA gene exhibit alterations in 3'-end processing and protein binding.
1987,
Pubmed
Chambers,
Association between the 7 S RNA and the lupus La protein varies among cell types.
1983,
Pubmed
Chan,
Human autoantibody-reactive epitopes of SS-B/La are highly conserved in comparison with epitopes recognized by murine monoclonal antibodies.
1987,
Pubmed
Cheng,
Spliceosome assembly in yeast.
1987,
Pubmed
Datta,
Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing.
1991,
Pubmed
Dreyfuss,
Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription.
1984,
Pubmed
Epstein,
The nucleotide sequence of nuclear U6 (4.7 S) RNA.
1980,
Pubmed
Francoeur,
Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro.
1982,
Pubmed
Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed
Gröning,
A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems.
1991,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Guthrie,
Spliceosomal snRNAs.
1988,
Pubmed
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hashimoto,
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
1984,
Pubmed
Hirai,
Uridylation of U6 RNA in a nuclear extract in Ehrlich ascites tumor cells.
1988,
Pubmed
Konarska,
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
1987,
Pubmed
Krol,
Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
1987,
Pubmed
,
Xenbase
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Lund,
In vitro synthesis of vertebrate U1 snRNA.
1989,
Pubmed
,
Xenbase
Lund,
Differential accumulation of U1 and U4 small nuclear RNAs during Xenopus development.
1987,
Pubmed
,
Xenbase
Lund,
Cyclic 2',3'-phosphates and nontemplated nucleotides at the 3' end of spliceosomal U6 small nuclear RNA's.
1992,
Pubmed
,
Xenbase
Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Mathews,
La antigen recognizes and binds to the 3'-oligouridylate tail of a small RNA.
1984,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Pruijn,
Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.
1991,
Pubmed
Reddy,
The capped U6 small nuclear RNA is transcribed by RNA polymerase III.
1987,
Pubmed
,
Xenbase
Reddy,
Identification of a La protein binding site in a RNA polymerase III transcript (4.5 I RNA).
1983,
Pubmed
Rinke,
Association of the lupus antigen La with a subset of U6 snRNA molecules.
1985,
Pubmed
Rinke,
Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies.
1982,
Pubmed
Shannon,
Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.
1991,
Pubmed
Singh,
Capping of mammalian U6 small nuclear RNA in vitro is directed by a conserved stem-loop and AUAUAC sequence: conversion of a noncapped RNA into a capped RNA.
1990,
Pubmed
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Terns,
Potential role of poly(A) polymerase in the assembly of polyadenylation-specific RNP complexes.
1991,
Pubmed
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Wu,
Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA.
1991,
Pubmed
