Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1979 Dec 01;7612:6448-52.
Show Gene links
Show Anatomy links
Synthesis and processing of maize storage proteins in Xenopus laevis oocytes.
Larkins BA
,
Pedersen K
,
Handa AK
,
Hurkman WJ
,
Smith LD
.
???displayArticle.abstract???
Xenopus oocytes injected with zein mRNAs efficiently synthesized maize storage proteins for prolonged periods. Under optimal conditions, zein was synthesized at 3 ng/hr and represented approximately 10% of the total protein synthesized in the oocyte. The mRNA from the normal maize inbred line directed synthesis of all the major zein components; however, products of mRNA from the opaque-2 mutant did not contain the largest zein component. Zein proteins synthesized in the oocyte were 2000 daltons smaller than proteins synthesized by cell-free translation of mRNAs in the wheat germ and reticulocyte systems. This result, which suggested that the oocyte processed prezein polypeptides into native zein proteins, was confirmed by amino-terminal sequence analysis of zein proteins from the oocytes. Cyanogen bromide cleavage of translation products from oocytes and the wheat germ system confirmed the existence of several proteins within each of the major zein components. However, we were unable to detect the presence of signal sequences on zein peptides by this technique.
Blobel,
Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
1975, Pubmed
Blobel,
Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
1975,
Pubmed
Burr,
Purification and translation of zein messenger RNA from maize endosperm protein bodies.
1978,
Pubmed
Eppig,
Amino acid pools in developing oocytes of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Gedamu,
Studies of the injection of poly(A)+ protamine mRNA into Xenopus laevis oocytes.
1978,
Pubmed
,
Xenbase
Jensen,
Locus number and multiple hemoglobins in Eutamias and Peromyscus.
1976,
Pubmed
Jones,
Reduced synthesis of zein in vitro by a high lysine mutant of maize.
1976,
Pubmed
Kindas-Mügge,
Insect protein synthesis in frog cells: the translation of honey bee promelittin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase
LaMarca,
Quantitative and qualitative analysis of RNA synthesis in stage 6 and stage 4 oocytes of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Larkins,
Synthesis and deposition of zein in protein bodies of maize endosperm.
1978,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase
Marcu,
Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.
1974,
Pubmed
Melcher,
In Vitro Synthesis of a Precursor to the Methionine-rich Polypeptide of the Zein Fraction of Corn.
1979,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Rapoport,
Synthesis of carp proinsulin in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Shih,
Kinetic analysis of amino acid pools and protein synthesis in amphibian oocytes and embryos.
1978,
Pubmed
,
Xenbase
Van der Donk,
Translation of plant messengers in egg cells of Xenopus laevis.
1975,
Pubmed
,
Xenbase
Vassart,
Thyroglobulin messenger RNA: translation of a 33-S mRNA into a peptide immunologically related to thyroglobulin.
1975,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Zehavi-Willner,
Subcellular compartmentation of albumin and globin made in oocytes under the direction of injected messenger RNA.
1977,
Pubmed
,
Xenbase
