Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Keryer G
,
Di Fiore B
,
Celati C
,
Lechtreck KF
,
Mogensen M
,
Delouvee A
,
Lavia P
,
Bornens M
,
Tassin AM
.
???displayArticle.abstract???
The small Ran GTPase, a key regulator of nucleocytoplasmic transport, is also involved in microtubule assembly and nuclear membrane formation. Herein, we show by immunofluorescence, immunoelectron microscopy, and biochemical analysis that a fraction of Ran is tightly associated with the centrosome throughout the cell cycle. Ran interaction with the centrosome is mediated by the centrosomal matrix A kinase anchoring protein (AKAP450). Accordingly, when AKAP450 is delocalized from the centrosome, Ran is also delocalized, and as a consequence, microtubule regrowth or anchoring is altered, despite the persisting association of gamma-tubulin with the centrosome. Moreover, Ran is recruited to Xenopus spermcentrosome during its activation for microtubule nucleation. We also demonstrate that centrosomal proteins such as centrin and pericentrin, but not gamma-tubulin, AKAP450, or ninein, undertake a nucleocytoplasmic exchange as they concentrate in the nucleus upon export inhibition by leptomycin B. Together, these results suggest a challenging possibility, namely, that centrosome activity could depend upon nucleocytoplasmic exchange of centrosomal proteins and local Ran-dependent concentration at the centrosome.
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989, Pubmed
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989,
Pubmed
Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed
Bobinnec,
Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells.
1998,
Pubmed
Bouckson-Castaing,
Molecular characterisation of ninein, a new coiled-coil protein of the centrosome.
1996,
Pubmed
Busson,
Dynein and dynactin are localized to astral microtubules and at cortical sites in mitotic epithelial cells.
1998,
Pubmed
Carazo-Salas,
Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly.
2001,
Pubmed
,
Xenbase
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Dasso,
The Ran GTPase: theme and variations.
2002,
Pubmed
Di Fiore,
Mammalian RanBP1 regulates centrosome cohesion during mitosis.
2003,
Pubmed
,
Xenbase
Doxsey,
Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
1994,
Pubmed
,
Xenbase
Fleig,
The fission yeast ran GTPase is required for microtubule integrity.
2000,
Pubmed
Flory,
Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin.
2000,
Pubmed
Félix,
Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation.
1994,
Pubmed
,
Xenbase
Gillingham,
The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin.
2000,
Pubmed
Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase
Guarguaglini,
Regulated Ran-binding protein 1 activity is required for organization and function of the mitotic spindle in mammalian cells in vivo.
2000,
Pubmed
,
Xenbase
Gönczy,
Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo.
1999,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Heald,
Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization.
1997,
Pubmed
,
Xenbase
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Joseph,
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
2002,
Pubmed
Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase
Keryer,
Dissociating the centrosomal matrix protein AKAP450 from centrioles impairs centriole duplication and cell cycle progression.
2003,
Pubmed
Keryer,
A high-affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II in the centrosome of human cells.
1993,
Pubmed
Klotz,
Parthenogenesis in Xenopus eggs requires centrosomal integrity.
1990,
Pubmed
,
Xenbase
Kudo,
Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region.
1999,
Pubmed
Künzler,
Targeting of Ran: variation on a common theme?
2001,
Pubmed
Martin,
Xgrip109: a gamma tubulin-associated protein with an essential role in gamma tubulin ring complex (gammaTuRC) assembly and centrosome function.
1998,
Pubmed
,
Xenbase
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Melchior,
Ran GTPase cycle: oOne mechanism -- two functions.
2001,
Pubmed
,
Xenbase
Merdes,
Formation of spindle poles by dynein/dynactin-dependent transport of NuMA.
2000,
Pubmed
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Mogensen,
Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein.
2000,
Pubmed
Mogensen,
Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells.
1997,
Pubmed
Mogensen,
Microtubule release and capture in epithelial cells.
1999,
Pubmed
Moore,
Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells.
2002,
Pubmed
,
Xenbase
Moudjou,
gamma-Tubulin in mammalian cells: the centrosomal and the cytosolic forms.
1996,
Pubmed
Murphy,
GCP5 and GCP6: two new members of the human gamma-tubulin complex.
2001,
Pubmed
Murphy,
The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p.
1998,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Nachury,
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
2001,
Pubmed
,
Xenbase
Ohba,
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
1999,
Pubmed
,
Xenbase
Piel,
The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells.
2000,
Pubmed
Quimby,
The interaction between Ran and NTF2 is required for cell cycle progression.
2000,
Pubmed
Ren,
Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis.
1993,
Pubmed
Richards,
The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1.
1995,
Pubmed
Roig,
Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.
2002,
Pubmed
Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed
Schroer,
Microtubules don and doff their caps: dynamic attachments at plus and minus ends.
2001,
Pubmed
Stearns,
Gamma-tubulin is a highly conserved component of the centrosome.
1991,
Pubmed
,
Xenbase
Stearns,
In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.
1994,
Pubmed
,
Xenbase
Takahashi,
Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the golgi apparatus.
1999,
Pubmed
Takahashi,
Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex.
2002,
Pubmed
Tassin,
Characterization of the human homologue of the yeast spc98p and its association with gamma-tubulin.
1998,
Pubmed
,
Xenbase
Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase
Wilde,
Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities.
2001,
Pubmed
,
Xenbase
Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase
Witczak,
Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450.
1999,
Pubmed
Wittmann,
TPX2, A novel xenopus MAP involved in spindle pole organization.
2000,
Pubmed
,
Xenbase
Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Zhang,
Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
