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Nucleic Acids Res
2000 May 15;2810:2187-93. doi: 10.1093/nar/28.10.2187.
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Hemicatenanes form upon inhibition of DNA replication.
Lucas I
,
Hyrien O
.
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Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, we have observed the formation of a strong spike of X-shaped DNA molecules in extracts that otherwise yield very little or no RIs. Formation of these joint molecules is also efficiently induced in replication-competent extracts upon inhibition of replication fork progression by aphidicolin. Although their electrophoretic properties are quite similar to those of Holliday junctions, 2D gels of doubly cut plasmids show that these junctions can link two plasmid molecules at any pair of DNA sequences, with no regard for sequence homology at the branch points. Neutral-neutral-alkaline 3D gels show that the junctions only contain single strands of parental size and no recombinant strands. A hemicatenane, in which one strand of a duplex is wound around one strand of another duplex, is the most likely structure to account for these observations. The mechanism of formation of these novel joint DNA molecules and their biological implications are discussed.
Bell,
Homologous association of chromosomal DNA during yeast meiosis.
1983, Pubmed
Bell,
Homologous association of chromosomal DNA during yeast meiosis.
1983,
Pubmed
Bell,
Separation of branched from linear DNA by two-dimensional gel electrophoresis.
1983,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Blow,
Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly.
1990,
Pubmed
,
Xenbase
Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed
Brown,
Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 topoisomerases.
1981,
Pubmed
Carroll,
DNA recombination and repair in oocytes, eggs, and extracts.
1991,
Pubmed
,
Xenbase
Collins,
Meiosis-specific formation of joint DNA molecules containing sequences from homologous chromosomes.
1994,
Pubmed
Dhar,
Replication initiates at multiple dispersed sites in the ribosomal DNA plasmid of the protozoan parasite Entamoeba histolytica.
1996,
Pubmed
DiGate,
Identification of a potent decatenating enzyme from Escherichia coli.
1988,
Pubmed
Ellis,
The Bloom's syndrome gene product is homologous to RecQ helicases.
1995,
Pubmed
Gangloff,
The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase.
1994,
Pubmed
Harmon,
RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination.
1999,
Pubmed
Hyrien,
Plasmid replication in Xenopus eggs and egg extracts: a 2D gel electrophoretic analysis.
1992,
Pubmed
,
Xenbase
Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase
Hyrien,
Mechanisms and consequences of replication fork arrest.
2000,
Pubmed
Kalejta,
Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.
1996,
Pubmed
Kmiec,
Homologous pairing of DNA molecules by Ustilago rec1 protein is promoted by sequences of Z-DNA.
1986,
Pubmed
Kobayashi,
Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I.
1998,
Pubmed
Laurie,
Geometry and physics of catenanes applied to the study of DNA replication.
1998,
Pubmed
Lehman,
Homologous and illegitimate recombination in developing Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase
Levac,
Inactivation of topoisomerase I or II may lead to recombination or to aberrant replication termination on both SV40 and yeast 2 micron DNA.
1996,
Pubmed
Liang,
Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method.
1994,
Pubmed
Lucas,
Mechanisms ensuring rapid and complete DNA replication despite random initiation in Xenopus early embryos.
2000,
Pubmed
,
Xenbase
Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Martín-Parras,
Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis.
1998,
Pubmed
,
Xenbase
Michel,
DNA double-strand breaks caused by replication arrest.
1997,
Pubmed
Rothstein,
The shuffling of a mortal coil.
1999,
Pubmed
Schwacha,
Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis.
1994,
Pubmed
Schwacha,
Identification of double Holliday junctions as intermediates in meiotic recombination.
1995,
Pubmed
Schwacha,
Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway.
1997,
Pubmed
Seigneur,
RuvAB acts at arrested replication forks.
1998,
Pubmed
Sogo,
Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.
1986,
Pubmed
Stewart,
rqh1+, a fission yeast gene related to the Bloom's and Werner's syndrome genes, is required for reversible S phase arrest.
1997,
Pubmed
Yu,
Positional cloning of the Werner's syndrome gene.
1996,
Pubmed
Zou,
Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism.
1997,
Pubmed
