Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes.
Kullberg R
,
Owens JL
,
Camacho P
,
Mandel G
,
Brehm P
.
???displayArticle.abstract???
Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of alpha-, beta-, gamma-, and delta-subunit mRNAs produced two predominant channel classes with conductances of approximately 50 and approximately 12 pS, while infrequent openings of approximately 25-pS channels were also observed. Injection of alpha-, beta-, and gamma-subunit mRNAs produced a single class of approximately 12-pS AcChoR channels, which resembled the smallest conductance channels present in alpha beta gamma omega-injected oocytes. Assembly of delta-less channels may thus explain the lowest conductance AcChoR channels in alpha beta gamma delta-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.
Auerbach,
Flickering of a nicotinic ion channel to a subconductance state.
1983, Pubmed
Auerbach,
Flickering of a nicotinic ion channel to a subconductance state.
1983,
Pubmed
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988,
Pubmed
,
Xenbase
Boulter,
Isolation of a clone coding for the alpha-subunit of a mouse acetylcholine receptor.
1985,
Pubmed
Buonanno,
A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit.
1986,
Pubmed
Colquhoun,
Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscle end-plate.
1985,
Pubmed
Dani,
Site-directed mutagenesis and single-channel currents define the ionic channel of the nicotinic acetylcholine receptor.
1989,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hamill,
Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells.
1981,
Pubmed
Henderson,
Single channel properties of newly synthesized acetylcholine receptors following denervation of mammalian skeletal muscle.
1987,
Pubmed
Imoto,
Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance.
1988,
Pubmed
,
Xenbase
LaPolla,
Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor.
1984,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Miledi,
Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Mishina,
Expression of functional acetylcholine receptor from cloned cDNAs.
,
Pubmed
,
Xenbase
Mishina,
Molecular distinction between fetal and adult forms of muscle acetylcholine receptor.
,
Pubmed
,
Xenbase
Morris,
Multiple conductance states of the acetylcholine receptor channel complex.
1986,
Pubmed
Owens,
Three conductance classes of nicotinic acetylcholine receptors are expressed in developing amphibian skeletal muscle.
1989,
Pubmed
,
Xenbase
Owens,
In vivo development of nicotinic acetylcholine receptor channels in Xenopus myotomal muscle.
1989,
Pubmed
,
Xenbase
Sakmann,
Role of acetylcholine receptor subunits in gating of the channel.
,
Pubmed
,
Xenbase
Sine,
Activation of a nicotinic acetylcholine receptor.
1984,
Pubmed
Takeda,
A patch-clamp study of the partial agonist actions of tubocurarine on rat myotubes.
1984,
Pubmed
White,
Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology.
1985,
Pubmed
,
Xenbase
Yoshii,
Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Yu,
Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression.
1986,
Pubmed
