Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes.
Jones NC
,
Richter JD
,
Weeks DL
,
Smith LD
.
???displayArticle.abstract???
The regulation of adenovirus type 5 gene expression by the E1a gene product was examined in microinjected Xenopus laevis oocytes. Chimeric genes were constructed which included the promoter region of early adenovirus type 5 gene 3 and the structural sequence which codes for the bacterial enzyme chloramphenicol-3-O-acetyltransferase (CAT). A plasmid containing this chimeric gene as well as plasmids containing the E1a gene were coinjected into oocyte nuclei. The presence of the E1a gene was shown to increase CAT activity by up to 8.5-fold over basal levels. Synthesis of the functional product from the E1a gene requires the removal of intron sequences by RNA splicing. The E1a gene and a derivative that precisely lacks the intron were equally effective in increasing CAT activity, suggesting that splicing of the primary E1a transcript is efficiently accomplished in the oocytenucleus. This was confirmed by directly examining the E1a mRNAs by the S1 mapping procedure. A protein extract from adenovirus type 5-infected HeLa cells enriched for the E1a protein may supplant the E1a plasmid in enhancing CAT activity. Synthesis of the CAT enzyme after gene injection is invariant in oocytes from the same frog, but oocytes from different frogs show a high degree of variability in their ability to synthesize the CAT enzyme. Microinjected X. laevis oocytes appear to be an extremely useful system to study the effects of protein elements on transcription.
Asselbergs,
Analysis of expression of adenovirus DNA (fragments) by microinjection in Xenopus oocytes. Independent synthesis of minor early region 2 proteins.
1983, Pubmed,
Xenbase
Asselbergs,
Analysis of expression of adenovirus DNA (fragments) by microinjection in Xenopus oocytes. Independent synthesis of minor early region 2 proteins.
1983,
Pubmed
,
Xenbase
Baker,
Promoters and heterogeneous 5' termini of the messenger RNAs of adenovirus serotype 2.
1981,
Pubmed
Banerji,
Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
1981,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Berk,
Structure of the adenovirus 2 early mRNAs.
1978,
Pubmed
Berk,
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
1979,
Pubmed
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Brown,
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Carlock,
Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species.
1981,
Pubmed
Chow,
Complex splicing patterns of RNAs from the early regions of adenovirus-2.
1979,
Pubmed
De Robertis,
Coupled transcription-translation of DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Dolecki,
Poly(A)+ RNA metabolism during oogenesis in Xenopus laevis.
1979,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eppig,
Amino acid pools in developing oocytes of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Etkin,
The synthesis of authentic sea urchin transcriptional and translational products by sea urchin histone genes injected into Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Gaynor,
Enhanced expression of adenovirus transforming proteins.
1982,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Graham,
Defective transforming capacity of adenovirus type 5 host-range mutants.
1978,
Pubmed
Gruss,
Simian virus 40 tandem repeated sequences as an element of the early promoter.
1981,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Jones,
Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
1979,
Pubmed
Jones,
An adenovirus type 5 early gene function regulates expression of other early viral genes.
1979,
Pubmed
Korn,
Oocyte extracts reactivate developmentally inert Xenopus 5S genes in somatic nuclei.
1982,
Pubmed
,
Xenbase
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
McKnight,
Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.
1980,
Pubmed
,
Xenbase
McKnight,
Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus.
1982,
Pubmed
Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase
Miller,
Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.
1982,
Pubmed
,
Xenbase
Montell,
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site.
1982,
Pubmed
Moreau,
The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
1981,
Pubmed
Nevins,
Mechanism of activation of early viral transcription by the adenovirus E1A gene product.
1981,
Pubmed
Nomura,
Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.
1980,
Pubmed
Osborne,
Far upstream initiation sites for adenovirus early region 1A transcription are utilized after the onset of viral DNA replication.
1983,
Pubmed
Perricaudet,
Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses.
1979,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Ricciardi,
Expression of early adenovirus genes requires a viral encoded acidic polypeptide.
1981,
Pubmed
Richter,
Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane-bound polysomes.
1981,
Pubmed
,
Xenbase
Richter,
Stimulation of Xenopus oocyte protein synthesis by microinjected adenovirus RNA.
1982,
Pubmed
,
Xenbase
Richter,
The recruitment of membrane-bound mRNAs for translation in microinjected Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Rungger,
DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Stunnenberg,
Bioassay for components regulating eukaryotic gene expression: a chromosomal factor involved in the generation of histone mRNA 3' termini.
1982,
Pubmed
,
Xenbase
Wasserman,
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Weeks,
E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.
1983,
Pubmed
Wickens,
Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei.
1980,
Pubmed
,
Xenbase
Wickens,
Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes.
1983,
Pubmed
,
Xenbase
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
