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Cloning and functional expression of a novel degenerin-like Na+ channel gene in mammals.
Sakai H
,
Lingueglia E
,
Champigny G
,
Mattei MG
,
Lazdunski M
.
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1. A degenerate polymerase chain reaction (PCR) homology screening procedure was applied to rat brain cDNA in order to identify novel genes belonging to the amiloride-sensitive Na+ channel and degenerin (NaC/DEG) family of ion channels. A single gene was identified that encodes a protein related to but clearly different from the already cloned members of the family (18-30 % amino acid sequence identity). Phylogenetic analysis linked this protein to the group of ligand-gated channels that includes the mammalian acid-sensing ion channels and the Phe-Met-Arg-Phe-amide (FMRFamide)-activated Na+ channel. 2. Expression of gain-of-function mutants after cRNA injection into Xenopus laevis oocytes or transient transfection of COS cells induced large constitutive currents. The activated channel was amiloride sensitive (IC50, 1.31 microM) and displayed a low conductance (9-10 pS) and a high selectivity for Na+ over K+ (ratio of the respective permeabilities, PNa+/PK+ >= 10), all of which are characteristic of NaC/DEG channel behaviour. 3. Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed a predominant expression of its mRNA in the small intestine, the liver (including hepatocytes) and the brain. This channel has been called the brain-liver-intestine amiloride-sensitive Na+ channel (BLINaC). 4. Corresponding gain-of-function mutations in Caenorhabditis elegans degenerins are responsible for inherited neurodegeneration in the nematode. Besides the BLINaC physiological function that remains to be established, mutations in this novel mammalian degenerin-like channel might be of pathophysiological importance in inherited neurodegeneration and liver or intestinal pathologies.
Adams,
Ripped pocket and pickpocket, novel Drosophila DEG/ENaC subunits expressed in early development and in mechanosensory neurons.
1998, Pubmed
Adams,
Ripped pocket and pickpocket, novel Drosophila DEG/ENaC subunits expressed in early development and in mechanosensory neurons.
1998,
Pubmed
Adams,
Protons activate brain Na+ channel 1 by inducing a conformational change that exposes a residue associated with neurodegeneration.
1998,
Pubmed
,
Xenbase
Barbry,
Molecular biology of Na+ absorption.
1997,
Pubmed
Barnard,
The transmitter-gated channels: a range of receptor types and structures.
1996,
Pubmed
Bassilana,
The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties.
1997,
Pubmed
,
Xenbase
Chalfie,
The identification and suppression of inherited neurodegeneration in Caenorhabditis elegans.
1990,
Pubmed
Champigny,
Mutations causing neurodegeneration in Caenorhabditis elegans drastically alter the pH sensitivity and inactivation of the mammalian H+-gated Na+ channel MDEG1.
1998,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cottrell,
The first peptide-gated ion channel.
1997,
Pubmed
,
Xenbase
Darboux,
dGNaC1, a gonad-specific amiloride-sensitive Na+ channel.
1998,
Pubmed
,
Xenbase
Darboux,
A new member of the amiloride-sensitive sodium channel family in Drosophila melanogaster peripheral nervous system.
1998,
Pubmed
Driscoll,
The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration.
1991,
Pubmed
Fink,
A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids.
1998,
Pubmed
,
Xenbase
García-Añoveros,
The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations.
1998,
Pubmed
,
Xenbase
Garty,
Epithelial sodium channels: function, structure, and regulation.
1997,
Pubmed
Gu,
Genetic interactions affecting touch sensitivity in Caenorhabditis elegans.
1996,
Pubmed
Hall,
Neuropathology of degenerative cell death in Caenorhabditis elegans.
1997,
Pubmed
Horisberger,
Amiloride-sensitive Na channels.
1998,
Pubmed
Huang,
Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans.
1994,
Pubmed
Ji,
Osmotic pressure regulates alpha beta gamma-rENaC expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Jurman,
Visual identification of individual transfected cells for electrophysiology using antibody-coated beads.
1994,
Pubmed
Lemieux,
A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes.
1992,
Pubmed
Lingueglia,
Cloning of the amiloride-sensitive FMRFamide peptide-gated sodium channel.
1995,
Pubmed
,
Xenbase
Liu,
Interaction between a putative mechanosensory membrane channel and a collagen.
1996,
Pubmed
Matsuda,
Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding.
1992,
Pubmed
McDonald,
Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel.
1995,
Pubmed
,
Xenbase
Price,
Cloning and expression of a novel human brain Na+ channel.
1996,
Pubmed
,
Xenbase
Sakai,
Endogenous arachidonic acid inhibits hypotonically-activated Cl- channels in isolated rat hepatocytes.
1996,
Pubmed
Take-Uchi,
An ion channel of the degenerin/epithelial sodium channel superfamily controls the defecation rhythm in Caenorhabditis elegans.
1998,
Pubmed
Tavernarakis,
unc-8, a DEG/ENaC family member, encodes a subunit of a candidate mechanically gated channel that modulates C. elegans locomotion.
1997,
Pubmed
Vandeyar,
A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.
1988,
Pubmed
Waldmann,
The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans.
1996,
Pubmed
Waldmann,
A proton-gated cation channel involved in acid-sensing.
1997,
Pubmed
,
Xenbase
Waldmann,
Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons.
1997,
Pubmed
Waldmann,
H(+)-gated cation channels: neuronal acid sensors in the NaC/DEG family of ion channels.
1998,
Pubmed
Wehner,
Hypertonic stress increases the Na+ conductance of rat hepatocytes in primary culture.
1995,
Pubmed
Wehner,
Hypertonicity-induced alkalinization of rat hepatocytes is not involved in activation of Na+ conductance or Na+,K+-ATPase.
1997,
Pubmed
Zhainazarov,
Single-channel currents of a peptide-gated sodium channel expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Zuo,
Neurodegeneration in Lurcher mice caused by mutation in delta2 glutamate receptor gene.
1997,
Pubmed
,
Xenbase
