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Hainaut P
,
Kowalski A
,
Giorgetti S
,
Baron V
,
Van Obberghen E
.
???displayArticle.abstract??? Insulin and insulin-like-growth-factor-I (IGF-I) receptors were partially purified from full-grown (stages V-VI) Xenopus laevis oocytes by affinity chromatography on wheat-germ agglutinin-agarose. Competitive-binding assays revealed high-affinity binding sites for both insulin and IGF-I (Kd = 2.5 x 10(-10) M and 8 x 10(-10) M respectively). However, IGF-I receptors were about 15 times more abundant than insulin receptors (22.5 x 10(11) versus 1.5 x 10(11)/mg of protein). Moreover, comparison of intact and collagenase-treated oocytes showed that most of the insulin receptors were in the oocyte envelopes, whereas IGF-I receptors were essentially at the oocyte surface. Oocyte receptors were composed of alpha-subunits of approximately 130 kDa and a doublet of beta-subunits of 95 and 105 kDa, which both had ligand-induced phosphorylation patterns compatible with IGF-I receptor beta-subunits. Accordingly, the receptor tyrosine kinase was stimulated at low IGF-I concentrations [half-maximally effective concentration (EC50) approximately 0.5-1 nM], and at higher insulin concentrations (EC50 approximately 20-50 nM). Partially purified glycoproteins from Xenopus liver and muscle contained mainly receptors of the insulin-receptor type, with alpha-subunits of 140 kDa in liver and 125 kDa in muscle, and doublets of beta-subunits of 92-98 kDa in liver and 85-94 kDa in muscle. Immunoprecipitation of receptors from oocytes, liver and muscle by receptor-specific anti-peptide antibodies suggested that the beta-subunit heterogeneity resulted from the existence of two distinct IGF-I receptors in oocytes and of two distinct insulin receptors in both liver and muscle. In the different tissues, the two receptor subtypes differed at least by their beta-subunit C-terminal region.
Alexandrides,
A novel fetal insulin-like growth factor (IGF) I receptor. Mechanism for increased IGF I- and insulin-stimulated tyrosine kinase activity in fetal muscle.
1989, Pubmed
Alexandrides,
A novel fetal insulin-like growth factor (IGF) I receptor. Mechanism for increased IGF I- and insulin-stimulated tyrosine kinase activity in fetal muscle.
1989,
Pubmed
Ballotti,
Antiphosphotyrosine antibodies modulate insulin receptor kinase activity and insulin action.
1989,
Pubmed
Baron,
Antibodies to insulin receptor tyrosine kinase stimulate its activity towards exogenous substrates without inducing receptor autophosphorylation.
1989,
Pubmed
Baron,
Insulin binding to its receptor induces a conformational change in the receptor C-terminus.
1990,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Braun,
Synthetic tyrosine polymers as substrates and inhibitors of tyrosine-specific protein kinases.
1984,
Pubmed
Corps,
Ligand-receptor interactions involved in the stimulation of Swiss 3T3 fibroblasts by insulin-like growth factors and insulin.
1988,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
DeMeyts,
Site-site interactions among insulin receptors. Characterization of the negative cooperativity.
1976,
Pubmed
Froesch,
Actions of insulin-like growth factors.
1985,
Pubmed
Gammeltoft,
Protein kinase activity of the insulin receptor.
1986,
Pubmed
Garofalo,
Insulin and insulinlike growth factor 1 (IGF-1) receptors during central nervous system development: expression of two immunologically distinct IGF-1 receptor beta subunits.
1989,
Pubmed
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Jonas,
Insulin-like growth factor binding to the atypical insulin receptors of a human lymphoid-derived cell line (IM-9).
1990,
Pubmed
Kellerer,
An altered IGF-I receptor is present in human leukemic cells.
1990,
Pubmed
King,
Direct demonstration of separate receptors for growth and metabolic activities of insulin and multiplication-stimulating activity (an insulinlike growth factor) using antibodies to the insulin receptor.
1980,
Pubmed
Kull,
Monoclonal antibodies to receptors for insulin and somatomedin-C.
1983,
Pubmed
Le Marchand-Brustel,
Functional labeling of insulin receptor subunits in live cells. Alpha 2 beta 2 species is the major autophosphorylated form.
1989,
Pubmed
Maller,
A study of the induction of cell division in amphibian oocytes by insulin.
1981,
Pubmed
,
Xenbase
Massagué,
The subunit structures of two distinct receptors for insulin-like growth factors I and II and their relationship to the insulin receptor.
1982,
Pubmed
Morgan,
Insulin action is blocked by a monoclonal antibody that inhibits the insulin receptor kinase.
1986,
Pubmed
,
Xenbase
Moxham,
Insulin-like growth factor I receptor beta-subunit heterogeneity. Evidence for hybrid tetramers composed of insulin-like growth factor I and insulin receptor heterodimers.
1989,
Pubmed
Poggi,
Effects and binding of insulin-like growth factor I in the isolated soleus muscle of lean and obese mice: comparison with insulin.
1979,
Pubmed
Rechler,
The nature and regulation of the receptors for insulin-like growth factors.
1985,
Pubmed
Rosen,
After insulin binds.
1987,
Pubmed
Rubin,
Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells.
1978,
Pubmed
Shuldiner,
Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective.
1989,
Pubmed
,
Xenbase
Shuldiner,
Isolation and characterization of two different insulins from an amphibian, Xenopus laevis.
1989,
Pubmed
,
Xenbase
Soos,
Immunological relationships between receptors for insulin and insulin-like growth factor I. Evidence for structural heterogeneity of insulin-like growth factor I receptors involving hybrids with insulin receptors.
1989,
Pubmed
Ullrich,
Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
,
Pubmed
Van Obberghen,
Biosynthetic labeling of insulin receptor: studies of subunits in cultured human IM-9 lymphocytes.
1981,
Pubmed
Vera,
Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
1989,
Pubmed
,
Xenbase
Yarden,
Growth factor receptor tyrosine kinases.
1988,
Pubmed
