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XB-ART-18906
Pflugers Arch 1995 Dec 01;4312:186-95. doi: 10.1007/bf00410190.
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Modulation of Na+ channel inactivation by the beta 1 subunit: a deletion analysis.

Chen C , Cannon SC .


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Na+ currents recorded from Xenopus oocytes expressing the Na+ channel alpha subunit alone inactivate with two exponential components. The slow component predominates in monomeric channels, while co-expression with the beta 1 subunit favors the fast component. Macropatch recordings show that the relative rates of these components are much greater than previously estimated from two-electrode measurements (approximately 30-fold vs approximately 5-fold). A re-assessment of steady-state inactivation, h infinity (V), shows that there is no depolarized shift of the slow component, provided a sufficiently long prepulse duration and repetition interval are used to achieve steady-state entry and recovery from inactivation, respectively. Deletion mutagenesis of the beta 1 subunit was used to define which regions of the subunit are required to modulate inactivation kinetics. The carboxy tail, comprising the entire predicted intracellular domain, can be deleted without a loss of activity; whereas small deletions in the extracellular amino domain or the signal peptide totally disrupt function.

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References [+] :
Bennett, A molecular basis for gating mode transitions in human skeletal muscle Na+ channels. 1993, Pubmed, Xenbase