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Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.
Korner J
,
Chun J
,
Harter D
,
Axel R
.
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We have combined gene cloning with an assay for prohormone biosynthesis and processing in Xenopus oocytes to identify the genes that encode mammalian prohormone processing enzymes. The coinjection of RNA encoding murine prohormone convertase 1 (mPC1), a mammalian endoprotease, along with proopiomelanocortin RNA into an oocyte results in the appropriate cleavage after paired basic residues in the proopiomelanocortin polyprotein necessary to generate corticotropin. The ability of mPC1 to generate corticotropin, along with the observation that mPC1 is specifically expressed in endocrine and neuronal cells, suggests that the mPC1 gene encodes the endopeptidase responsible for the pathway of proopiomelanocortincleavage observed in the anterior pituitary.
Bresnahan,
Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.
1990, Pubmed
Bresnahan,
Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.
1990,
Pubmed
Douglass,
Polyprotein gene expression: generation of diversity of neuroendocrine peptides.
1984,
Pubmed
Eipper,
Structure and biosynthesis of pro-adrenocorticotropin/endorphin and related peptides.
1980,
Pubmed
Enoch,
Activation of the human beta-interferon gene requires an interferon-inducible factor.
1986,
Pubmed
Estell,
Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.
1985,
Pubmed
Fuller,
Intracellular targeting and structural conservation of a prohormone-processing endoprotease.
1989,
Pubmed
Fuller,
Enzymes required for yeast prohormone processing.
1988,
Pubmed
Germain,
The yeast KEX-2-processing endoprotease is active in the Golgi apparatus of transfected NIH 3T3 fibroblasts.
1990,
Pubmed
Ikemura,
In vitro processing of pro-subtilisin produced in Escherichia coli.
1988,
Pubmed
Jacobs,
Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis.
1985,
Pubmed
Julius,
Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor.
1984,
Pubmed
Julius,
Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway.
1984,
Pubmed
Kennedy,
Sequencing of proteins from two-dimensional gels by using in situ digestion and transfer of peptides to polyvinylidene difluoride membranes: application to proteins associated with sensitization in Aplysia.
1988,
Pubmed
Kraut,
Serine proteases: structure and mechanism of catalysis.
1977,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Kurjan,
Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
1982,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Matsudaira,
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
1987,
Pubmed
Mizuno,
Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases.
1988,
Pubmed
Moore,
Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation.
1983,
Pubmed
Nakanishi,
A family of glutamate receptor genes: evidence for the formation of heteromultimeric receptors with distinct channel properties.
1990,
Pubmed
Power,
Secretion and autoproteolytic maturation of subtilisin.
1986,
Pubmed
Pustell,
Interactive molecular biology computing.
1988,
Pubmed
Roebroek,
Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein.
1986,
Pubmed
Rosen,
After insulin binds.
1987,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Santerre,
Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.
1981,
Pubmed
Schalken,
fur gene expression as a discriminating marker for small cell and nonsmall cell lung carcinomas.
1987,
Pubmed
Schnabel,
Proteolytic processing of pro-ACTH/endorphin begins in the Golgi complex of pituitary corticotropes and AtT-20 cells.
1989,
Pubmed
Seidah,
Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2.
1991,
Pubmed
Seidah,
cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases.
1990,
Pubmed
Smeekens,
Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.
1991,
Pubmed
Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase
Sossin,
Cellular and molecular biology of neuropeptide processing and packaging.
1989,
Pubmed
Thim,
Secretion and processing of insulin precursors in yeast.
1986,
Pubmed
Thomas,
Yeast KEX2 endopeptidase correctly cleaves a neuroendocrine prohormone in mammalian cells.
1988,
Pubmed
Uhler,
Complete amino acid sequence of mouse pro-opiomelanocortin derived from the nucleotide sequence of pro-opiomelanocortin cDNA.
1983,
Pubmed
Wells,
Subtilisin--an enzyme designed to be engineered.
1988,
Pubmed
Wise,
Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site.
1990,
Pubmed
van den Ouweland,
Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2.
1990,
Pubmed
