Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1990 Dec 01;913:4391-8. doi: 10.1002/j.1460-2075.1990.tb07889.x.
Show Gene links
Show Anatomy links
Sequence and functional expression of a single alpha subunit of an insect nicotinic acetylcholine receptor.
Marshall J
,
Buckingham SD
,
Shingai R
,
Lunt GG
,
Goosey MW
,
Darlison MG
,
Sattelle DB
,
Barnard EA
.
???displayArticle.abstract???
We report the isolation and sequence of a cDNA clone that encodes a locust (Schistocerca gregaria) nervous system nicotinic acetylcholine receptor (AChR) subunit (alpha L1). The calculated molecular weight of the unglycosylated polypeptide, which contains in the proposed extracellular domain two adjacent cysteine residues which are characteristic of alpha (ligand binding) subunits, is 60,641 daltons. Injection into Xenopus oocytes, of RNA synthesized from this clone in vitro, results in expression of functional nicotinic receptors in the oocyte membrane. In these, nicotine opens a cation channel; the receptors are blocked by both alpha-bungarotoxin (alpha-Bgt) and kappa-bungarotoxin (kappa-Bgt). Reversible block of the expressed insect AChR by mecamylamine, d-tubocurarine, tetraethylammonium, bicuculline and strychnine has also been observed. These data are entirely consistent with previously reported electrophysiological studies on in vivo insect nicotinic receptors and also with biochemical studies on an alpha-Bgt affinity purified locust AChR. Thus, a functional receptor exhibiting the characteristic pharmacology of an in vivo insect nicotinic AChR can be expressed in Xenopus oocytes by injection with a single subunit RNA.
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987, Pubmed
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987,
Pubmed
Bertrand,
Activation and blocking of neuronal nicotinic acetylcholine receptor reconstituted in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Blagburn,
Internal presynaptic tetraethylammonium (TEA+) blocks cholinergic transmission at a synapse between identified neurones.
1987,
Pubmed
Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988,
Pubmed
,
Xenbase
Bossy,
Conservation of neural nicotinic acetylcholine receptors from Drosophila to vertebrate central nervous systems.
1988,
Pubmed
Boulter,
Isolation of a clone coding for the alpha-subunit of a mouse acetylcholine receptor.
1985,
Pubmed
Boulter,
Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family.
1987,
Pubmed
,
Xenbase
Boulter,
Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit.
,
Pubmed
Breer,
Molecular forms and subunit structure of the acetylcholine receptor in the central nervous system of insects.
1985,
Pubmed
Buonanno,
A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit.
1986,
Pubmed
Chiappinelli,
Kappa-bungarotoxin blocks nicotinic transmission at an identified invertebrate central synapse.
1989,
Pubmed
Deneris,
Beta 3: a new member of nicotinic acetylcholine receptor gene family is expressed in brain.
1989,
Pubmed
Deneris,
Primary structure and expression of beta 2: a novel subunit of neuronal nicotinic acetylcholine receptors.
1988,
Pubmed
,
Xenbase
Drummond,
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Duvoisin,
The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4.
1989,
Pubmed
,
Xenbase
Filbin,
Partial purification and characterisation of an acetylcholine receptor with nicotinic properties from the supraoesophageal ganglion of the locust (Schistocerca gregaria).
1983,
Pubmed
Finer-Moore,
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.
1984,
Pubmed
Freeman,
Effect of alpha-bungarotoxin on retinotectal synaptic transmission in the goldfish and the toad.
1980,
Pubmed
Goldman,
Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous system.
1987,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Hanke,
Channel properties of an insect neuronal acetylcholine receptor protein reconstituted in planar lipid bilayers.
,
Pubmed
Hermans-Borgmeyer,
Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila.
1986,
Pubmed
Kao,
Acetylcholine receptor binding site contains a disulfide cross-link between adjacent half-cystinyl residues.
1986,
Pubmed
Kao,
Identification of the alpha subunit half-cystine specifically labeled by an affinity reagent for the acetylcholine receptor binding site.
1984,
Pubmed
Lane,
Differential accessibility to two insect neurones does not account for differences in sensitivity to alpha-bungarotoxin.
1982,
Pubmed
Lindstrom,
Molecular studies of the neuronal nicotinic acetylcholine receptor family.
1987,
Pubmed
Loring,
Characterization of neuronal nicotinic receptors by snake venom neurotoxins.
1988,
Pubmed
Marshall,
Cloning of putative nicotinic acetylcholine receptor genes from the locust.
1988,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Nef,
Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
1988,
Pubmed
Neumann,
Mapping of the alpha-bungarotoxin binding site within the alpha subunit of the acetylcholine receptor.
1986,
Pubmed
Noda,
Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.
1983,
Pubmed
Parker,
A slowly inactivating potassium current in native oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Pinnock,
Kappa-bungarotoxin blocks an alpha-bungarotoxin-sensitive nicotinic receptor in the insect central nervous system.
1988,
Pubmed
Pritchett,
Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sattelle,
GABA receptors on the cell-body membrane of an identified insect motor neuron.
1988,
Pubmed
Schloss,
Neuronal acetylcholine receptors in Drosophila: the ARD protein is a component of a high-affinity alpha-bungarotoxin binding complex.
1988,
Pubmed
Schmieden,
Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor.
1989,
Pubmed
,
Xenbase
Snyder,
Neurotransmitter receptors in the brain: biochemical identification.
1976,
Pubmed
Sontheimer,
Functional chloride channels by mammalian cell expression of rat glycine receptor subunit.
1989,
Pubmed
Wada,
Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor.
1988,
Pubmed
,
Xenbase
Wafford,
gamma-Aminobutyric acid-activated 36Cl- influx: a functional in vitro assay for CNS gamma-aminobutyric acid receptors of insects.
1987,
Pubmed
White,
Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology.
1985,
Pubmed
,
Xenbase
de la Garza,
Selective antagonism of nicotine actions in the rat cerebellum with alpha-bungarotoxin.
1987,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
