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Nucleic Acids Res
1998 Jul 01;2613:3208-14. doi: 10.1093/nar/26.13.3208.
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Cap ribose methylation of c-mos mRNA stimulates translation and oocyte maturation in Xenopus laevis.
Kuge H
,
Brownlee GG
,
Gershon PD
,
Richter JD
.
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In Xenopus oocytes, progesterone stimulates the cytoplasmic polyadenylation and resulting translational activation of c-mos mRNA, which is necessary for the induction of oocyte maturation. Although details of the biochemistry of polyadenylation are beginning to emerge, the mechanism by which 3' poly(A) addition stimulates translation initiation is enigmatic. A previous report showed that polyadenylation induced cap-specific 2'-O-methylation, and suggested that this 5' end modification was important for translational activation. Here, we demonstrate that injected c-mos RNA undergoes polyadenylation and cap ribose methylation. Inhibition of this methylation by S-isobutylthioadenosine (SIBA), a methyltransferase inhibitor, has little effect on progesterone-induced c-mos mRNA polyadenylation or general protein synthesis, but prevents the synthesis of Mos protein as well as oocyte maturation. Maturation can be rescued, however, by the injection of factors that act downstream of Mos, such as cyclin A and B mRNAs. Most importantly, we show that the translational efficiency of injected mRNAs containing cap-specific 2'-O-methylation (cap I) is significantly enhanced compared to RNAs that do not contain the methylated ribose (cap 0). These results suggest that cap ribose methylation of c-mos mRNA is important for translational recruitment and for the progression of oocytes through meiosis.
Bilger,
Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements.
1994,
Pubmed
,
Xenbase
Brownlee,
Solid phase synthesis of 5'-diphosphorylated oligoribonucleotides and their conversion to capped m7Gppp-oligoribonucleotides for use as primers for influenza A virus RNA polymerase in vitro.
1995,
Pubmed
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Galili,
Role of the 3'-poly(A) sequence in translational regulation of mRNAs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed
Gebauer,
Synthesis and function of Mos: the control switch of vertebrate oocyte meiosis.
1997,
Pubmed
,
Xenbase
Gershon,
Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus.
1991,
Pubmed
Gershon,
Stimulation of poly(A) tail elongation by the VP39 subunit of the vaccinia virus-encoded poly(A) polymerase.
1993,
Pubmed
Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Hake,
Specificity of RNA binding by CPEB: requirement for RNA recognition motifs and a novel zinc finger.
1998,
Pubmed
,
Xenbase
Hodel,
Specific protein recognition of an mRNA cap through its alkylated base.
1997,
Pubmed
Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed
Kuge,
Cytoplasmic 3' poly(A) addition induces 5' cap ribose methylation: implications for translational control of maternal mRNA.
1995,
Pubmed
,
Xenbase
Marcotrigiano,
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP.
1997,
Pubmed
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
Minich,
Chromatographic resolution of in vivo phosphorylated and nonphosphorylated eukaryotic translation initiation factor eIF-4E: increased cap affinity of the phosphorylated form.
1994,
Pubmed
Moore,
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites.
1992,
Pubmed
Munns,
Characterization of antibodies specific for N6-methyladenosine and for 7-methylguanosine.
1977,
Pubmed
Munroe,
mRNA poly(A) tail, a 3' enhancer of translational initiation.
1990,
Pubmed
Muthukrishnan,
Influence of 5'-terminal cap structure on the initiation of translation of vaccinia virus mRNA.
1978,
Pubmed
Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase
Richter,
Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane-bound polysomes.
1981,
Pubmed
,
Xenbase
Robert-Géro,
Inhibition of virus-induced cell transformation by synthetic analogues of S-adenosyl homocysteine.
1975,
Pubmed
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Schnierle,
Cap-specific mRNA (nucleoside-O2'-)-methyltransferase and poly(A) polymerase stimulatory activities of vaccinia virus are mediated by a single protein.
1992,
Pubmed
Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase
Stebbins-Boaz,
Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.
1994,
Pubmed
,
Xenbase
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
,
Xenbase
Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
Xenbase
