Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The centrosomal kinase Aurora A (AurA) is required for cell cycle progression, centrosome maturation and spindle assembly. However, the way it participates in spindle assembly is still quite unclear. Using the Xenopus egg extract system, we have dissected the role of AurA in the different microtubule (MT) assembly pathways involved in spindle formation. We developed a new tool based on the activation of AurA by TPX2 to clearly define the requirements for localization and activation of the kinase during spindle assembly. We show that localized AurA kinase activity is required to target factors involved in MT nucleation and stabilization to the centrosome, therefore promoting the formation of a MT aster. In addition, AurA strongly enhances MT nucleation mediated by the Ran pathway through cytoplasmic phosphorylation. Altogether, our data show that AurA exerts an effect as a key regulator of MT assembly during M phase and therefore of bipolar spindle formation.
Albee,
Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin beta.
2006, Pubmed,
Xenbase
Albee,
Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin beta.
2006,
Pubmed
,
Xenbase
Barr,
Aurora-A: the maker and breaker of spindle poles.
2007,
Pubmed
Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase
Bayliss,
Determinants for Aurora-A activation and Aurora-B discrimination by TPX2.
2004,
Pubmed
,
Xenbase
Brunet,
Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Carazo-Salas,
Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly.
2001,
Pubmed
,
Xenbase
De Luca,
A functional interplay between Aurora-A, Plk1 and TPX2 at spindle poles: Plk1 controls centrosomal localization of Aurora-A and TPX2 spindle association.
2006,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Gadea,
Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin.
2006,
Pubmed
,
Xenbase
Gergely,
Centrosomal TACCtics.
2002,
Pubmed
,
Xenbase
Girdler,
Validating Aurora B as an anti-cancer drug target.
2006,
Pubmed
Groen,
XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly.
2004,
Pubmed
,
Xenbase
Gruss,
The mechanism of spindle assembly: functions of Ran and its target TPX2.
2004,
Pubmed
Haydon,
Identification of novel phosphorylation sites on Xenopus laevis Aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography.
2003,
Pubmed
,
Xenbase
Hoar,
MLN8054, a small-molecule inhibitor of Aurora A, causes spindle pole and chromosome congression defects leading to aneuploidy.
2007,
Pubmed
Kelly,
Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly.
2007,
Pubmed
,
Xenbase
Kinoshita,
Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
2005,
Pubmed
,
Xenbase
Koffa,
HURP is part of a Ran-dependent complex involved in spindle formation.
2006,
Pubmed
,
Xenbase
Kufer,
Human TPX2 is required for targeting Aurora-A kinase to the spindle.
2002,
Pubmed
,
Xenbase
Peset,
Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosis.
2005,
Pubmed
,
Xenbase
Stenoien,
Dynamic association of a tumor amplified kinase, Aurora-A, with the centrosome and mitotic spindle.
2003,
Pubmed
,
Xenbase
Tsai,
Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly.
2005,
Pubmed
,
Xenbase
Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase
Zhang,
Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity.
2008,
Pubmed
,
Xenbase
