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Plant Physiol
2008 Oct 01;1482:856-69. doi: 10.1104/pp.108.123844.
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AtPTR1 and AtPTR5 transport dipeptides in planta.
Komarova NY
,
Thor K
,
Gubler A
,
Meier S
,
Dietrich D
,
Weichert A
,
Suter Grotemeyer M
,
Tegeder M
,
Rentsch D
.
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Transporters for di- and tripeptides belong to the large and poorly characterized PTR/NRT1 (peptide transporter/nitrate transporter 1) family. A new member of this gene family, AtPTR5, was isolated from Arabidopsis (Arabidopsis thaliana). Expression of AtPTR5 was analyzed and compared with tissue specificity of the closely related AtPTR1 to discern their roles in planta. Both transporters facilitate transport of dipeptides with high affinity and are localized at the plasma membrane. Mutants, double mutants, and overexpressing lines were exposed to several dipeptides, including toxic peptides, to analyze how the modified transporter expression affects pollen germination, growth of pollen tubes, root, and shoot. Analysis of atptr5 mutants and AtPTR5-overexpressing lines showed that AtPTR5 facilitates peptide transport into germinating pollen and possibly into maturating pollen, ovules, and seeds. In contrast, AtPTR1 plays a role in uptake of peptides by roots indicated by reduced nitrogen (N) levels and reduced growth of atptr1 mutants on medium with dipeptides as the sole N source. Furthermore, overexpression of AtPTR5 resulted in enhanced shoot growth and increased N content. The function in peptide uptake was further confirmed with toxic peptides, which inhibited growth. The results show that closely related members of the PTR/NRT1 family have different functions in planta. This study also provides evidence that the use of organic N is not restricted to amino acids, but that dipeptides should be considered as a N source and transport form in plants.
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003, Pubmed
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003,
Pubmed
Bidlingmeyer,
Rapid analysis of amino acids using pre-column derivatization.
1984,
Pubmed
Bock,
Integrating membrane transport with male gametophyte development and function through transcriptomics.
2006,
Pubmed
Campalans,
Identification of differentially expressed genes by the cDNA-AFLP technique during dehydration of almond (Prunus amygdalus).
2001,
Pubmed
Carter,
The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.
2004,
Pubmed
Chiang,
Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2.
2004,
Pubmed
,
Xenbase
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Cunningham,
Kinetic Analysis of Protein Degradation by a Freshwater Wetland Sediment Community.
1989,
Pubmed
Dietrich,
AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis.
2004,
Pubmed
,
Xenbase
Dohmen,
An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
1991,
Pubmed
Dunkley,
Mapping the Arabidopsis organelle proteome.
2006,
Pubmed
Footitt,
The COMATOSE ATP-binding cassette transporter is required for full fertility in Arabidopsis.
2007,
Pubmed
Frommer,
Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters.
1994,
Pubmed
Gatz,
Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants.
1992,
Pubmed
Gifford,
Crop productivity and photoassimilate partitioning.
1984,
Pubmed
Glass,
The regulation of nitrate and ammonium transport systems in plants.
2002,
Pubmed
Grallath,
The AtProT family. Compatible solute transporters with similar substrate specificity but differential expression patterns.
2005,
Pubmed
Higgins,
Peptide transport by germinating barley embryos: Uptake of physiological di- and oligopeptides.
1978,
Pubmed
Higgins,
The Peptide pools of germinating barley grains: relation to hydrolysis and transport of storage proteins.
1981,
Pubmed
Hirner,
Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll.
2006,
Pubmed
Hirner,
Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.
1998,
Pubmed
Honys,
Transcriptome analysis of haploid male gametophyte development in Arabidopsis.
2004,
Pubmed
Jamai,
Proton-Peptide Co-Transport in Broad Bean Leaf Tissues.
1994,
Pubmed
Jeong,
A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.
2004,
Pubmed
,
Xenbase
Karim,
AtPTR3, a wound-induced peptide transporter needed for defence against virulent bacterial pathogens in Arabidopsis.
2007,
Pubmed
Karim,
Structural and functional characterization of AtPTR3, a stress-induced peptide transporter of Arabidopsis.
2005,
Pubmed
Kaye,
Competition for nitrogen between plants and soil microorganisms.
1997,
Pubmed
Kwart,
Differential expression of two related amino acid transporters with differing substrate specificity in Arabidopsis thaliana.
1993,
Pubmed
Lee,
AAP1 transports uncharged amino acids into roots of Arabidopsis.
2007,
Pubmed
Lee,
Selective expression of a novel high-affinity transport system for acidic and neutral amino acids in the tapetum cells of Arabidopsis flowers.
2004,
Pubmed
Meyer,
AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana.
2006,
Pubmed
,
Xenbase
Miranda,
Peptide and amino acid transporters are differentially regulated during seed development and germination in faba bean.
2003,
Pubmed
Pate,
Nutrition of a developing legume fruit: functional economy in terms of carbon, nitrogen, water.
1977,
Pubmed
Paungfoo-Lonhienne,
Plants can use protein as a nitrogen source without assistance from other organisms.
2008,
Pubmed
Pina,
Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation.
2005,
Pubmed
Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed
Rentsch,
Transporters for uptake and allocation of organic nitrogen compounds in plants.
2007,
Pubmed
Röber,
Synthesis of fructans in tubers of transgenic starch-deficient potato plants does not result in an increased allocation of carbohydrates.
1996,
Pubmed
Schmidt,
The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana.
2007,
Pubmed
Schulze,
Transporters for ammonium, amino acids and peptides are expressed in pitchers of the carnivorous plant Nepenthes.
1999,
Pubmed
Segonzac,
Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter.
2007,
Pubmed
Shimaoka,
Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.
2004,
Pubmed
Song,
Antisense expression of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants.
1997,
Pubmed
Stacey,
The Arabidopsis AtOPT3 protein functions in metal homeostasis and movement of iron to developing seeds.
2008,
Pubmed
Stacey,
AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in Arabidopsis.
2002,
Pubmed
Svennerstam,
Comprehensive screening of Arabidopsis mutants suggests the lysine histidine transporter 1 to be involved in plant uptake of amino acids.
2007,
Pubmed
Tsay,
Nitrate transporters and peptide transporters.
2007,
Pubmed
Voll,
The Arabidopsis phenylalanine insensitive growth mutant exhibits a deregulated amino acid metabolism.
2004,
Pubmed
WRIGHT,
Amino acid uptake by plant roots.
1962,
Pubmed
Waterworth,
The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane.
2000,
Pubmed
,
Xenbase
West,
Cloning and functional characterisation of a peptide transporter expressed in the scutellum of barley grain during the early stages of germination.
1998,
Pubmed
,
Xenbase
Williams,
TRANSPORTERS RESPONSIBLE FOR THE UPTAKE AND PARTITIONING OF NITROGENOUS SOLUTES.
2001,
Pubmed
Woody,
The WiscDsLox T-DNA collection: an arabidopsis community resource generated by using an improved high-throughput T-DNA sequencing pipeline.
2007,
Pubmed
Xiang,
A mini binary vector series for plant transformation.
1999,
Pubmed
Zimmermann,
GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox.
2004,
Pubmed
