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Translation and stability of human globin mRNA in Xenopus oocytes.
Maniatis GM
,
Ramirez F
,
Cann A
,
Marks PA
,
Bank A
.
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Human globin messenger RNA (mRNA) prepared from erythroid cells of patients with sickle cell anemia has been translated in Xenopus laevis oocytes. Addition of hemin to the injected mRNA causes total globin synthesis to increase and the ratio of alpha- to betas-globin synthesis (alpha/betas ratio) to approach unity. To determine the effect of the length of the poly-(A) segment on human globin mRNA stability, 10 S globin mRNA was fractionated into poly-(A)-poor fractions by oligo (dT)-cellulose column chromatography. When oocytes are injected with each of these fractions, translation of the poly-(A)-rich globin mRNA is sustained for a longer period than that of the poly-(A)-poor mRNA. Regardless of the mRNA fraction injected, the alpha/betas ratio of the synthesized globin decreases as the injected oocytes are incubated for longer periods. The results indicate that in frog oocytes poly-(A)-rich mRNA has greater translational stability than poly-(A)-poor mRNA, AND beta-mRNA has greater stability than alpha-mRNA with comparable poly-(A) content.
Adamson,
Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesis.
1972, Pubmed
Adamson,
Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesis.
1972,
Pubmed
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase
Bank,
Regulation of globin synthesis in the thalassemias.
1974,
Pubmed
Beuzard,
Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.
1973,
Pubmed
Beuzard,
The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.
1974,
Pubmed
Cann,
Polyadenylate sequences and biologic activity of human globin messenger ribonucleic acid.
1974,
Pubmed
Chan,
Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations.
1976,
Pubmed
,
Xenbase
Clegg,
Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).
1966,
Pubmed
FANELLI,
Studies on the structure of hemoglobin. I. Physicochemical properties of human globin.
1958,
Pubmed
Giglioni,
Translational control of globin synthesis by haemin in Xenopus oocytes.
1973,
Pubmed
,
Xenbase
Gorski,
Size heterogeneity of polyadenylate sequences in mouse globin messenger RNA.
1974,
Pubmed
Grayzel,
The stimulation of globin synthesis by heme.
1966,
Pubmed
Gurdon,
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
1973,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Hieter,
Stabilization of an RNA molecule by 3'-terminal poly (A)-induced inhibition of RNase activity.
1976,
Pubmed
Housman,
Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.
1973,
Pubmed
Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase
Lane,
Control of translation of globin mRNA in embryonic cells.
1974,
Pubmed
Lane,
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
1971,
Pubmed
Lodish,
Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.
1971,
Pubmed
Mathews,
Specificity of the control of protein synthesis by haemin.
1973,
Pubmed
Maxwell,
Hemin control of globin synthesis: an assay for the inhibitor formed in the absence of hemin and some characteristics of its formation.
1971,
Pubmed
Metafora,
Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.
1972,
Pubmed
Nienhuis,
Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.
1971,
Pubmed
Nudel,
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
1976,
Pubmed
,
Xenbase
Sheiness,
Polyadenylic acid segment in mRNA becomes shorter with age.
1973,
Pubmed
Sheiness,
Possible relationship of poly(A) shortening to mRNA turnover.
1975,
Pubmed
Sippel,
Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.
1974,
Pubmed
Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed
Stevens,
Specific IgG mRNA molecules from myeloma cells in heterogeneous nuclear and cytoplasmic RNA containing poly-A.
1972,
Pubmed
,
Xenbase
Williamson,
Globin messenger sequences in high molecular weight RNA from embryonic mouse liver.
1973,
Pubmed
,
Xenbase
Williamson,
Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.
1974,
Pubmed
Yip,
Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.
1975,
Pubmed
,
Xenbase
Zucker,
The synthesis of globin dimers by a reticulocyte cell-free system.
1967,
Pubmed
