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Fig. 1.
Light inactivation of GluN1/GluN2B NMDARs incorporating a genetically encoded photoactive UAA. (A, Left) Four plasmids encoding the GluN1 subunit with an amber stop codon at position Y109 (red dot), the wt GluN2 subunit, the suppressor tRNA (Yam), and the engineered tRNA synthetase (RS) were coinjected into Xenopus oocytes. (Center) Crystal structure of the GluA2 AMPA receptor (40). The three major domains—N-terminal domain (NTD), agonist-binding domain (ABD) and transmembrane domain (TMD)—are arranged in layers. One NTD dimer is highlighted. (Right) Crystal structure of the NMDAR GluN1/GluN2B NTD heterodimer (16); the ifenprodil molecule is omitted for clarity. LL, lower lobe; UL, upper lobe. The GluN1-Y109 site is highlighted. On UV irradiation, the azide moiety generates a biradical, which subsequently can react with a nearby residue to form a covalent adduct. (B) Current amplitudes from oocytes injected with plasmids as indicated, in the absence or presence of UAAs. For each condition, 20 oocytes were tested. Only currents >10 nA were plotted. (C) Representative current traces showing UV-induced current inhibition of GluN1-Y109AzF/GluN2B receptors but not wt GluN1/GluN2B receptors. (D) UV-induced current modifications at wt GluN1/GluN2B (1.11 ± 0.13; n = 8), GluN1-Y109AzF/GluN2Bwt with (0.28 ± 0.05; n = 16) or without (0.31 ± 0.05; n = 5) agonist, and GluN1-Y109Bpa/GluN2Bwt (1.15 ± 0.06; n = 5) receptors. Error bars, SD. (E) MK-801 inhibition kinetics of wt GluN1/GluN2B and GluN1-Y109AzF/GluN2B receptors before and after UV treatment.
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Fig. 2.
UV-induced functional changes at the GluN1-Y109 position are GluN2 NTD-specific. (Left) Representative current traces of UV-induced effects on receptors incorporating GluN1-Y109AzF and wt GluN2B, GluN2A-2BNTD, GluN2A-2BUL-loop (the loop represents GluN2B-208MSLDDGD), or wt GluN2A. (Right) Relative currents (Iuv/I0) from receptors containing the GluN1-Y109AzF subunits and 2B (0.28 ± 0.05; n = 16), 2A-2BNTD (0.43 ± 0.08; n = 6), 2A-2BUL-loop (0.78 ± 0.04; n = 5), or 2A (1.10 ± 0.22; n = 5) subunit. Error bars, SD.
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Fig. 3.
Structural basis for the differential GluN1/GluN2 NTD dimer interfaces. (A and B, Left) View of the UL/UL interface in the GluN1/GluN2B NTD dimer crystal structure (A) (16) or GluN1/GluN2A NTD dimer model (B). Residues mutated into cysteines are represented in sticks with Cα-Cα distances indicated in Å. (Right) Immunoblots from Xenopus oocytes expressing either wt or mutant subunits. M1 indicates the GluN1 monomer (∼110 kDa); M2 the GluN2A or GluN2B monomer (∼180 kDa); D1/1 the GluN1 homodimer (∼220 kDa); and D1/2 the GluN1/GluN2 heterodimer (∼290 kDa). Treatment with (+) or without (−) DTE is also shown. *, a nonspecific band; n.i., noninjected oocytes. (C) Electrostatic interactions at the GluN1-UL/GluN2-LL interface as observed in the GluN1/GluN2B NTD dimer crystal structure (distances indicated in Å). (D and E) Relative MK-801 inhibition kinetics of receptors incorporating wt or mutant GluN1 and GluN2 subunits. Values are (from left to right) in D, 1.00 ± 0.11, 1.05 ± 0.06, 0.90 ± 0.07, 0.92 ± 0.09, 0.81 ± 0.12, 0.90 ± 0.06, and 0.79 ± 0.07; in E, 1.00 ± 0.13, 0.66 ± 0.07, 0.34 ± 0.03, 0.63 ± 0.12; 0.38 ± 0.03, 0.54 ± 0.07, 0.43 ± 0.03, 0.28 ± 0.04, and 0.85 ± 0.08. n = 4–35. *P < 0.05, **P < 0.01, ***P < 0.001, Student t test. Error bars, SD.
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Fig. 4.
Exploration of the photo–cross-linking site. (A) Local environment around the GluN1-Y109 site in the GluN1/GluN2B NTD dimer (16). The potential candidates on the GluN1-KSIH-loop for UV cross-linking are highlighted, and distances are indicated in Å. The interface residues GluN1-K322 and GluN2B-D206 are also highlighted. (B) Changes in current amplitude after UV illumination on GluN1-Y109AzF/GluN2B wt receptors or receptors incorporating an additional substitution (L, V, A, or G) at position GluN1-I133. Values are 0.28 ± 0.05, 0.28 ± 0.02, 0.37 ± 0.05, 0.48 ± 0.09, and 0.82 ± 0.08; n = 3–16. (Inset) Normalized current traces for GluN1-I133 and its substitutions. (C) The GluN1-133 residue volume and the UV-induced current reductions are strongly correlated (linear regression, R2 = 0.89). (D) Disruption of the UV effect by destabilizing the GluN1-UL/GluN2B-LL electrostatic contacts. Values are (from left to right): 0.28 ± 0.05, 0.32 ± 0.05, 0.40 ± 0.03, 0.28 ± 0.03, 0.85 ± 0.09, 0.43 ± 0.08, and 0.40 ± 0.04. n = 4–16. *P < 0.05, ***P < 0.001, Student t test. Error bars, SD.
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Fig. 5.
Expression of UV-sensitive GluN1-Y109AzF NMDARs in mammalian cells. (A) UV-induced current modifications at GluN1 wt/GFP-Y182AzF-GluN2B (1.03 ± 0.12, n = 6) or GluN1-Y109AzF/GFP-Y182AzF-GluN2B (0.69 ± 0.25, n = 7) receptors expressed in HEK cells. (B, Right) UV-induced current modifications at GluN1-N616R/GFP-Y182AzF-GluN2B (1.27 ± 0.45, n = 7) or GluN1-Y109AzF-N616R/GFP-Y182AzF-GluN2B (0.84 ± 0.19, n = 9) receptors expressed in culture hippocampal neurons. (Left) Fluorescence images of neurons transfected with three plasmids encoding GluN1-Y109amb-N616R, GFP-Y182amb-GluN2B, and the suppressor tRNA/AzFRS pair (SI Appendix, Fig. S10A) with (Upper) or without (Lower) AzF in the medium. *P < 0.05, Student t test.
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Fig. S1. GluN1-Y109 mutants alter both receptor channel activity and pharmacology.
(A) X-ray crystal structure of the GluN1/GluN2B NTD dimer (pdb code: 3QEL; ref. (11)). The GluN1 subunit is
colored in light blue, and GluN2B subunit in yellow. Zoom up view of the UL/UL interface. Residue GluN1-Y109 is
highlighted in purple spheres. (B) Relative inhibition kinetics by 50 nM MK-801 of receptors incorporating wildtype (wt) GluN2B subunits and GluN1 wt (1.0 ± 0.1, n=19), GluN1-Y109A (0.44 ± 0.06, n=6), GluN1-Y109C (1.04
± 0.11, n=3), GluN1-Y109R (5.87 ± 0.29, n=3), GluN1-Y109E (7.33 ± 0.51, n=4). ***P<0.001, Student’s t-test. (C)
Proton sensitivity of GluN2B receptors containing wild-type or mutant GluN1 subunits. Values of pHIC50 and nH are:
7.40 ± 0.03 and 1.6 for GluN1 wt (n=3), 7.38 ± 0.02 and 1.5 for GluN1-Y109C (n=2), 6.52 ± 0.06 and 1.0 for
GluN1-Y109A (n=3). (D) Zinc dose-response curves of GluN2B receptors incorporating wild-type or mutant GluN1
subunits. IC50 (nM) and nH are: 576 and 0.9 for GluN1 wt, 452 and 0.9 for GluN1-Y109C, 1829 and 1.2 for GluN1-
Y109A. Maximum inhibition was fixed as 1. n=3-4 for each group. (E) 100 µM spermine potentiation of GluN2B
receptors incorporating wild-type or mutant GluN1 subunits (experiments done at extracellular pH 6.6). Values of
current potentiation are: 5.38 ± 0.42 for GluN1wt, 3.53 ± 0.24 for GluN1-Y109C, 0.94 ± 0.03 for GluN1-Y109A.
n=3 for each group. Error bars represent the S.D.
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Fig. S2. Effect of UV treatment on the gating and pharmacological properties of GluN1-Y109AzF
or GluN1-Y109Bpa incorporated receptors.
(A) Relative MK-801 inhibition kinetics from oocytes expressing either wt GluN1/GluN2B receptors (1.00 ± 0.07;
n=7), GluN1-Y109AzF/GluN2B receptors before (1.05 ± 0.11; n=7) and after (2.69 ± 0.48; n=9) UV treatment, or
GluN1-Y109Bpa/GluN2B receptors before (3.32 ± 0.37; n=6) and after (2.91 ± 0.16; n=4) UV treatment. Error
bars, S.D. (B) Ifenprodil sensitivity of wt GluN1/GluN2B receptors and GluN1-Y109AzF/GluN2B receptors before
and after UV treatment. IC50 (µM), Hill coefficients (nH), maximal inhibition values are, respectively: 0.23 ± 0.02,
1.38, 0.90 for wt GluN1/GluN2B receptors; 0.34 ± 0.04, 1.18, 0.95 for GluN1-Y109AzF/GluN2B before UV, and
2.35 ± 0.34, 0.81, 0.95 after UV. n=3-4 for each group. (C) Zinc dose-response curves for wt GluN1/GluN2B
receptors (IC50 = 0.68 μM, nH = 0.83, and Inhibitionmax = 0.98), GluN1-Y109AzF/GluN2B wt receptors before (IC50
= 0.58 μM, nH = 0.89, and Inhibitionmax = 1.00) and after (IC50 = 0.77 μM, nH = 0.82, and Inhibitionmax = 0.98) UV
treatment. n=3-4 for each group. (D) Glutamate dose-response curves for wt GluN1/GluN2B receptors before
(EC50 =1.27 μM, nH =1.52) and after UV (EC50 = 1.24 μM, nH = 1.69); for GluN1-Y109AzF/GluN2B wt receptors
before (EC50 = 1.77 μM, nH = 1.78) and after (EC50 = 0.96 μM, nH = 1.40) UV treatment. n=3 for each group. (E)
Glycine dose-response curves for wt GluN1/GluN2B receptors before (EC50 = 0.44 μM, nH = 2.3) and after UV
(EC50 = 0.47 μM, nH = 2.3); for the GluN1-Y109AzF/GluN2B wt receptors before (EC50 = 0.39 μM, nH = 2.1) and
after (EC50 = 0.46 μM, nH = 2.00) UV treatment. n = 3-4 for each group. Error bars, S.D
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Fig. S3. Genetically encoding AzF in GluN1/GluN2A receptors
(A) Representative dot plot of the current amplitudes from oocytes injected with plasmids encoding GluN1-
Y109amb (40 ng/μl), GluN2A-wt (40 ng/μl), Yam suppressor tRNA (5 ng/μl), and AzF-RS (1ng/μl) in the absence
(empty circles) or presence (solid circles) of the UAA in the incubation media. For each condition, 20 oocytes
were tested. Only currents >10 nA were plotted. (B) Relative inhibition kinetics by 10 nM MK-801 applied to
oocytes expressing wild-type (wt) GluN1/GluN2A receptors (1.00 ± 0.24), GluN1-Y109AzF/GluN2A before (1.33 ±
0.26) and after (1.29 ± 0.14) UV treatment. n=3 for each group. Error bars represent the S.D.
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Fig. S4. Modulating UV- and chemical-induced receptor alterations by GluN2A and GluN2B NTD
chimeras
(A) UV modification of current carried by receptors containing GluN1-Y109AzF subunits and GluN2A wt (1.12 ±
0.24), GluN2B (0.28 ± 0.05), GluN2A-2BUL (1.06 ± 0.07), GluN2B-2ANTD (1.16 ± 0.06), GluN2B-2A(NTD+L)
(1.11 ± 0.09) or GluN2A-2B(NTD+L) (0.68 ± 0.08). n=3-16 for each group. (B) MTSEA modification at GluN1-
Y109C receptors containing various GluN2 subunits. Values are: GluN2A wt (0.70 ± 0.08), GluN2B wt (0.13 ±
0.05), GluN2A-2BUL (0.52 ± 0.04), GluN2B-2ANTD (0.59 ± 0.01), GluN2B-2A(NTD+L) (0.58 ± 0.13), GluN2A2B(NTD+L) (0.23 ± 0.03). n = 3-12 for each group. Error bars represent the S.D.
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Fig. S5. Subunit specific changes in receptor activity induced by MTSEA binding to the GluN1-
Y109C mutant.
(A) MTS reagent current modifications at GluN2B receptors containing either wild-type (wt) GluN1 or GluN1-
Y109C mutant subunits. Values from left to right are: 0.95 ± 0.04, 0.13 ± 0.05 for MTSEA; 0.91 ± 0.02, 0.69 ± 0.01
for MTSET; 0.82 ± 0.07, 0.78 ± 0.04 for MTS-PtrEA, 0.74 ± 0.01, 0.76 ± 0.01 for MTSES. n=3-8 for each group.
(B) Left panel: UV modification of current carried by receptors containing GluN1-Y109AzF subunits and GluN2A
wt (1.12 ± 0.24), GluN2B (0.28 ± 0.05), GluN2A-2BUL (1.06 ± 0.07), GluN2B-2ANTD (1.16 ± 0.06), GluN2B2A(NTD+L) (1.11 ± 0.09) or GluN2A-2B(NTD+L) (0.68 ± 0.08). n=3-16 for each group. Right panel: MTSEA
modification at GluN1-Y109C receptors containing various GluN2 subunits. Values are: GluN2A wt (0.70 ± 0.08),
GluN2B wt (0.13 ± 0.05), GluN2A-2BUL (0.52 ± 0.04), GluN2B-2ANTD (0.59 ± 0.01), GluN2B-2A(NTD+L) (0.58 ±
0.13), GluN2A-2B(NTD+L) (0.23 ± 0.03). n = 3-12 for each group. Error bars represent the S.D. ***P<0.001,
Student’s t-test. Only the MTS-specific irreversible component of the inhibitory effects on receptor activity is taken
into account for the calculation of relative currents (see SI Material and Methods).
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Fig. S6. Differential NTD UL/UL interface in GluN2A- and GluN2B- containing receptors.
(A,B) Immunoblots from Xenopus oocytes expressing either wt or mutant GluN1 and GluN2B subunits detected
by anti-GluN1 (blue) or anti-GluN2B (yellow) antibodies. M1 indicates the expected band location of the GluN1
monomer (~110 kDa); M2 indicates the expected band location of the GluN2B monomer (~180 kDa); D1/2
indicates the expected band location of the GluN1/GluN2B heterodimer (~290 kDa); D1/1 indicates the expected
band location of the GluN1 homodimer (~220 kDa). * indicates a non-specific band. n.i. indicates non-injected
oocytes. Treatment with the reducing reagent DTE is also marked (+DTE). (C,D) Immunoblots from Xenopus
oocytes expressing either wild-type (wt) or mutant GluN1 and wt or mutant GluN2A subunits detected by antiGluN1 (blue) or anti-GluN2A (orange) antibodies. M1 indicates the expected band location of the GluN1 monomer
(~110 kDa); M2 indicates the expected band location of the GluN2A monomer (~180 kDa); D1/1 indicates the
expected band location of the GluN1 homodimer (~220 kDa). The anti-GluN1 antibodies MAB363 and MAB1586
were used in (A,C) and (D), respectively.
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Fig. S7. Engineering a disulfide bond at the GluN1 UL/GluN2 LL NTD dimer interface affects
receptor activity.
(A) 3D homology model of the GluN1/GluN2A NTD heterodimer based on GluN1/GluN2B NTD crystal structure
(pdb code: 3QEL; ref. (11)). The putative interface formed by the GluN1 NTD upper lobe (UL) and the GluN2A
NTD lower lobe (LL) is boxed. Inset: sphere representation of the residues mutated into cysteines: GluN1-L320
(purple) and GluN2A-E211 (orange). (B) Sequence alignment in the region around the GluN1 UL/GluN2 LL
interface. Residues mutated into cysteines are indicated with asterisks (GluN1-L320 and GluN2A-E211). The
residues contributing to the electrostatic interactions are colored in purple for GluN1 (K131, K322 and R323),
orange for GluN2A (D207 and E211) and yellow for GluN2B (D206, D210 and D213), respectively. (C)
Immunoblots from Xenopus oocytes expressing GluN1/GluN2A receptors incorporating either wild-type (wt) or
cysteine mutant subunits. The GluN1 monomer band (M1) is ~110 kDa, while the GluN2A monomer band (M2) is
~180 kDa. The D1/2 arrowheads indicate the GluN1/GluN2A disulfide-linked heterodimer (absent in DTE). (D)
Relative MK801 inhibition kinetics of GluN1/GluN2A receptors incorporating wt or cysteine mutant subunits. Inset:
Close-up view of the modelled GluN1 UL/GluN2A LL NTD dimer interface. Mean values are, from left to right:
1.00 ± 0.06 (n=9), 0.93 ± 0.09 (n=6), 0.98 ± 0.07 (n=6), and the double-cysteine receptors before, 1.98 ± 0.14
(n=9), and after (black bar), 0.74 ± 0.07 (n=6), DTE treatment. (E) Relative MK801 inhibition kinetics of
GluN1/GluN2B receptors incorporating wt or cysteine mutant subunits. GluN1* represents the GluN1-C744AC798A mutant subunit (9, 12). Inset: Close-up view of the GluN1 UL/GluN2B LL NTD dimer interface as seen in
the GluN1/GluN2B NTD dimer crystal structure (11). The two introduced cysteines, GluN1-L320C and GluN2BD210C are indicated in sphere. Mean values are, from left to right: 1.00 ± 0.12 (n=9), 0.89 ± 0.11 (n=7), 0.81 ±
0.15 (n=10), and the double cysteine containing receptors before, 3.45 ± 0.50 (n=6), and after (black bar), 0.53 ±
0.06 (n=5), DTE treatment. *P<0.05, ***P<0.001, Student’s t-test. Error bars represent the S.D.
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Fig. S8. Electrostatic interactions at the GluN1-UL/GluN2B-LL NTD dimer interface.
Relative MK-801 inhibition kinetics of receptors incorporating wild-type (wt) or mutant GluN1, and wt or mutant
GluN2B subunits. Values from left to right are: GluN1 wt/GluN2B wt (1.00 ± 0.13; n=35), GluN1-3D/GluN2B wt
(0.66 ± 0.09; n=8), GluN1wt/GluN2B-2K (referring to D206K-D210K; 0.35 ± 0.04; n=8), GluN1 wt/GluN2B-3K
(0.28 ± 0.04; n=9), GluN1 wt/GluN2B-S208A-3K (0.33 ± 0.07; n=6), GluN1-3D/GluN2B-3K (0.45 ± 0.03; n=4),
GluN1-R115A-3D/GluN2B-3K (0.85 ± 0.08; n=8), GluN1-3D/GluN2B-S208A-3K (0.72 ± 0.05; n=2), GluN1-R115A3D/GluN2B-S208A-3K (0.78 ± 0.08; n=6). Error bars represent the S.D.
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Fig. S9. No heterodimer formation after UV-treatment of GluN1-Y109AzF/GluN2B receptors.
Western blots from Xenopus oocytes expressing wild-type (wt) GluN1/GluN2B or GluN1-Y109AzF/GluN2B
receptors probed either with anti-GluN1 (left) or anti-GluN2B (right) antibodies. GluN1 monomers (M1, ~110 kDa)
or GluN2B monomers (M2, ~180 kDa) are indicated with arrowheads. The - and + signs refer to the absence or
presence, respectively, of UV irradiation (365 nm, 30 min). n.i. indicates non-injected oocytes.
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Fig. S10. Optimization and validation in Xenopus oocytes of molecular biology constructs for
expression of AzF containing NMDARs in mammalian cells
(A) Plasmid constructions designed for mammalian cell transfections. One plasmid encodes the GluN1 subunit
with the Y109 site mutated to the amber stop codon and the N616 at the Q/R/N pore site mutated to R in order to
confer resistance to extracellular Mg2+ block. Another plasmid encodes the GFP fused to the N-terminal of the
GluN2B subunit. The amber stop codon was inserted at the Y182 position of the GPF, in order to use green GFP
fluorescence as a reporter of AzF incorporation. One bidirectional plasmid named pET21-tRNA-AzFRS encodes
the suppressor tRNA Yam driven by its internal promoter and the AzFRS driven by the CMV promoter. (B)
Current amplitudes from oocytes co-injected with GluN1-Y109amb, wild-type (wt) GluN2B and pET21-tRNAAzFRS plasmids, incubated in the absence (n=9) or presence (n=15) of the UAA AzF. Only currents >10 nA were
plotted. (C) UV-induced current modifications at GluN1 wt/GluN2B wt (1.11 ± 0.13; n=8), GluN1-Y109AzF/GluN2B
wt (0.28 ± 0.05; n=16), GluN1-Y109AzF/GFP-GluN2B (0.31 ± 0.03; n=5), and GluN1-Y109AzF-N616R/GFPY182AzF-GluN2B receptors (0.54 ± 0.05; n=5). Error bars represent the S.D.
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