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Proc Natl Acad Sci U S A
1996 Aug 20;9317:9200-5. doi: 10.1073/pnas.93.17.9200.
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Cloning, expression, and distribution of a Ca(2+)-activated K+ channel beta-subunit from human brain.
Tseng-Crank J
,
Godinot N
,
Johansen TE
,
Ahring PK
,
Strøbaek D
,
Mertz R
,
Foster CD
,
Olesen SP
,
Reinhart PH
.
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We have cloned and expressed a Ca(2+)-activated K+ channel beta-subunit from human brain. The open reading frame encodes a 191-amino acid protein possessing significant homology to a previously described subunit cloned from bovine muscle. The gene for this subunit is located on chromosome 5 at band q34 (hslo-beta). There is no evidence for alternative RNA splicing of this gene product. hslo-beta mRNA is abundantly expressed in smooth muscle, but expression levels are low in most other tissues, including brain. Brain subregions in which beta-subunit mRNA expression is relatively high are the hippocampus and corpus callosum. The coexpression of hslo-beta mRNA together with hslo-alpha subunits in either Xenopus oocytes or stably transfected HEK 293 cells give rise to Ca(2+)-activated potassium currents with a much increased calcium and/or voltage sensitivity. These data indicate that the beta-subunit shows a tissue distribution different to that of the alpha-subunit, and in many tissues there may be no association of alpha-subunits with beta-subunits. These beta-subunits can play a functional role in the regulation of neuronal excitability by tuning the Ca2+ and/or the voltage dependence of alpha-subunits.
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,
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,
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,
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,
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Hamill,
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Hicks,
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,
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Isom,
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Knaus,
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Latorre,
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Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro.
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Marty,
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Maruyama,
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,
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,
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,
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McPhalen,
Calcium-binding sites in proteins: a structural perspective.
1991,
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Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
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,
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Moczydlowski,
Gating kinetics of Ca2+-activated K+ channels from rat muscle incorporated into planar lipid bilayers. Evidence for two voltage-dependent Ca2+ binding reactions.
1983,
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Morales,
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,
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Pierce,
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,
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Reinhart,
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1989,
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Reinhart,
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1991,
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Reinhart,
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1995,
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Reinhart,
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Rettig,
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1994,
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,
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Rhodes,
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1995,
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Roberts,
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Robitaille,
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Robitaille,
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,
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