Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1995 Oct 01;1510:5412-22. doi: 10.1128/MCB.15.10.5412.
Show Gene links
Show Anatomy links
Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.
Chen J
,
Tomkinson AE
,
Ramos W
,
Mackey ZB
,
Danehower S
,
Walter CA
,
Schultz RA
,
Besterman JM
,
Husain I
.
???displayArticle.abstract???
Three biochemically distinct DNA ligase activities have been identified in mammalian cell extracts. We have recently purified DNA ligase II and DNA ligase III to near homogeneity from bovine liver and testistissue, respectively. Amino acid sequencing studies indicated that these enzymes are encoded by the same gene. In the present study, human and murine cDNA clones encoding DNA ligase III were isolated with probes based on the peptide sequences. The human DNA ligase III cDNA encodes a polypeptide of 862 amino acids, whose sequence is more closely related to those of the DNA ligases encoded by poxviruses than to replicative DNA ligases, such as human DNA ligase I. In vitro transcription and translation of the cDNA produced a catalytically active DNA ligase similar in size and substrate specificity to the purified bovine enzyme. The DNA ligase III gene was localized to human chromosome 17, which eliminated this gene as a candidate for the cancer-prone disease Bloom syndrome that is associated with DNA joining abnormalities. DNA ligase III is ubiquitously expressed at low levels, except in the testes, in which the steady-state levels of DNA ligase III mRNA are at least 10-fold higher than those detected in other tissues and cells. Since DNA ligase I mRNA is also present at high levels in the testes, we examined the expression of the DNA ligase genes during spermatogenesis. DNA ligase I mRNA expression correlated with the contribution of proliferating spermatogonia cells to the testes, in agreement with the previously defined role of this enzyme in DNA replication. In contrast, elevated levels of DNA ligase III mRNA were observed in primary spermatocytes undergoing recombination prior to the first meiotic division. Therefore, we suggest that DNA ligase III seals DNA strand breaks that arise during the process of meiotic recombination in germ cells and as a consequence of DNA damage in somatic cells.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Badley,
A simple, rapid method for the purification of poly A+ RNA.
1988,
Pubmed
Bardwell,
Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.
1992,
Pubmed
Barnes,
Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae.
1990,
Pubmed
Barnes,
Mutations in the DNA ligase I gene of an individual with immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.
1992,
Pubmed
Bellvé,
Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.
1977,
Pubmed
Brown,
Evidence that approximately eighty per cent of the soluble proteins from Ehrlich ascites cells are Nalpha-acetylated.
1976,
Pubmed
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994,
Pubmed
,
Xenbase
Chaganti,
A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes.
1974,
Pubmed
Chan,
DNA-ligase activities appear normal in the CHO mutant EM9.
1984,
Pubmed
Chan,
Altered DNA ligase I activity in Bloom's syndrome cells.
,
Pubmed
Colinas,
A DNA ligase gene in the Copenhagen strain of vaccinia virus is nonessential for viral replication and recombination.
1990,
Pubmed
Cooper,
Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision.
1993,
Pubmed
Creissen,
Regulation of DNA ligase activity by poly(ADP-ribose).
1982,
Pubmed
Dillehay,
The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.
1983,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Fujimoto,
Changes in polyadenylation of lactate dehydrogenase-X mRNA during spermatogenesis in mice.
1988,
Pubmed
Goyon,
Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase.
1993,
Pubmed
Hake,
Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse.
1990,
Pubmed
Henderson,
Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges.
1985,
Pubmed
Higashitani,
Purification of DNA ligases from mouse testis and their behavior during meiosis.
1990,
Pubmed
Higgins,
CLUSTAL V: improved software for multiple sequence alignment.
1992,
Pubmed
Husain,
Purification and characterization of DNA ligase III from bovine testes. Homology with DNA ligase II and vaccinia DNA ligase.
1995,
Pubmed
,
Xenbase
Jessberger,
A mammalian protein complex that repairs double-strand breaks and deletions by recombination.
1993,
Pubmed
Johnston,
Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.
1978,
Pubmed
Johnston,
The expression in meiosis of genes which are transcribed periodically in the mitotic cell cycle of budding yeast.
1986,
Pubmed
Kerr,
Vaccinia DNA ligase complements Saccharomyces cerevisiae cdc9, localizes in cytoplasmic factories and affects virulence and virus sensitivity to DNA damaging agents.
1991,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Lehman,
DNA ligase: structure, mechanism, and function.
1974,
Pubmed
Lönn,
Altered formation of DNA replication intermediates in human 46 BR fibroblast cells hypersensitive to 3-aminobenzamide.
1989,
Pubmed
Lönn,
An abnormal profile of DNA replication intermediates in Bloom's syndrome.
1990,
Pubmed
McDaniel,
Elevated sister chromatid exchange phenotype of Bloom syndrome cells is complemented by human chromosome 15.
1992,
Pubmed
Nasmyth,
Temperature-sensitive lethal mutants in the structural gene for DNA ligase in the yeast Schizosaccharomyces pombe.
1977,
Pubmed
Nasmyth,
Genetic and enzymatic characterization of conditional lethal mutants of the yeast Schizosaccharomyces pombe with a temperature-sensitive DNA ligase.
1979,
Pubmed
Padmore,
Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae.
1991,
Pubmed
Petrini,
A wild-type DNA ligase I gene is expressed in Bloom's syndrome cells.
1991,
Pubmed
Prigent,
Activation of mammalian DNA ligase I through phosphorylation by casein kinase II.
1992,
Pubmed
Prigent,
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
1994,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Roberts,
Different active sites of mammalian DNA ligases I and II.
1994,
Pubmed
,
Xenbase
Rzhetsky,
Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference.
1992,
Pubmed
Rünger,
Joining of linear plasmid DNA is reduced and error-prone in Bloom's syndrome cells.
1989,
Pubmed
Saiki,
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Savini,
Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme.
1994,
Pubmed
Siciliano,
Assignment of a human DNA-repair gene associated with sister-chromatid exchange to chromosome 19.
1986,
Pubmed
Smith,
Transcriptional mapping and nucleotide sequence of a vaccinia virus gene encoding a polypeptide with extensive homology to DNA ligases.
1989,
Pubmed
Stubbs,
DNA synthesis at selective sites during pachytene in mouse spermatocytes.
1986,
Pubmed
Sym,
ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis.
1993,
Pubmed
Szostak,
The double-strand-break repair model for recombination.
1983,
Pubmed
Söderhäll,
DNA ligases during rat liver regeneration.
1976,
Pubmed
Söderhäll,
Mammalian DNA ligases. Serological evidence for two separate enzymes.
1975,
Pubmed
Takahashi,
Two distinct DNA ligases from Drosophila melanogaster embryos.
1987,
Pubmed
Tomkinson,
DNA ligase III is the major high molecular weight DNA joining activity in SV40-transformed human fibroblasts: normal levels of DNA ligase III activity in Bloom syndrome cells.
1993,
Pubmed
,
Xenbase
Tomkinson,
Location of the active site for enzyme-adenylate formation in DNA ligases.
1991,
Pubmed
Tomkinson,
Three distinct DNA ligases in mammalian cells.
1991,
Pubmed
,
Xenbase
Waga,
Reconstitution of complete SV40 DNA replication with purified replication factors.
1994,
Pubmed
Walter,
Testis and somatic Xrcc-1 DNA repair gene expression.
1994,
Pubmed
Wang,
Mammalian DNA ligase II is highly homologous with vaccinia DNA ligase. Identification of the DNA ligase II active site for enzyme-adenylate formation.
1994,
Pubmed
Willis,
DNA ligase I deficiency in Bloom's syndrome.
,
Pubmed
Willis,
Structural alterations of DNA ligase I in Bloom syndrome.
1987,
Pubmed
