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XB-ART-38699
J Vis Exp 2008 Sep 02;18:. doi: 10.3791/891.
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Preparation and fractionation of Xenopus laevis egg extracts.

Cross MK , Powers M .


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Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

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