Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Restoration of binding of oxidized transcription factor IIIA to 5S RNA by thioredoxin.
Pastori RL
,
Zucker KE
,
Xing YY
.
???displayArticle.abstract???
7S particles from Xenopus oocytes were completely dissociated under non-reducing conditions. Studies using glycerol gradient centrifugation show that unlike the native 7S particle in which 5S RNA and TFIIIA co-sedimented in a fairly sharp peak, the RNA from the denatured 7S sedimented at the position corresponding to the 5S RNA and the TFIIIA sedimented as a wide peak between 6S and 12S. Thioredoxin from E. coli can catalyze the reactivation of the TFIIIA as measured by its ability to reform the 7S particle. The rate of reactivation with thioredoxin was significantly greater than with dithiothreitol. Oxidized thioredoxin was unable to reactivate TFIIIA. Pure TFIIIA can be inactivated and subsequently reactivated in the same way by formation of a cross-linked structure via intermolecular disulfide bridges.
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984, Pubmed,
Xenbase
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Creighton,
Disulfide bonds as probes of protein folding pathways.
1986,
Pubmed
Dignam,
Eukaryotic gene transcription with purified components.
1983,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Frankel,
Metal-dependent folding of a single zinc finger from transcription factor IIIA.
1987,
Pubmed
Garner,
Stoichiometry of catabolite activator protein/adenosine cyclic 3',5'-monophosphate interactions at the lac promoter of Escherichia coli.
1982,
Pubmed
Grippo,
Proof that the endogenous, heat-stable glucocorticoid receptor-activating factor is thioredoxin.
1985,
Pubmed
Hanas,
Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.
1983,
Pubmed
,
Xenbase
Hanas,
Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.
1983,
Pubmed
,
Xenbase
Hanas,
Xenopus transcription factor A promotes DNA reassociation.
1985,
Pubmed
,
Xenbase
Hazuda,
DNA-activated ATPase activity associated with Xenopus transcription factor A.
1986,
Pubmed
,
Xenbase
Holmgren,
Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide.
1979,
Pubmed
Huber,
Use of the cytotoxic nuclease alpha-sarcin to identify the binding site on eukaryotic 5 S ribosomal ribonucleic acid for the ribosomal protein L5.
1986,
Pubmed
,
Xenbase
Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase
Krust,
The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors.
1986,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase
Pigiet,
Thioredoxin-catalyzed refolding of disulfide-containing proteins.
1986,
Pubmed
Romaniuk,
Characterization of the RNA binding properties of transcription factor IIIA of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase
