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Control of 5S RNA transcription in Xenopus somatic cell chromatin: activation with an oocyte extract.
Reynolds WF
,
Bloomer LS
,
Gottesfeld JM
.
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A chromatin fraction enriched for Xenopus 5S RNA genes has been isolated by restriction endonuclease digestion and sucrose gradient velocity sedimentation. Soluble chromatin sedimenting at 70-80S contains approximately 50% of the oocyte-expressed 5S RNA genes and only 1.5-3% of total chromatin DNA; this represents a 15- to 30-fold purification of the 5S genes. Such chromatin isolated from somatic cells (blood and cultured kidney cells) retains the transcriptionally-inactive state of the oocyte-expressed 5S genes. Soluble chromatin from somatic cells prepared by micrococcal nuclease digestion also retains the inactive state of the oocyte-type 5S genes. It is likely that the level of chromatin structure responsible for inactivity of the oocyte genes in somatic cells is the nucleosome or short chains of nucleosomes and not supranucleosomal structures. The oocyte-type genes can be rendered transcriptionally active in somatic cell chromatin either by salt extraction of some chromosomal proteins or by treatment with the ion exchange resin Dowex A50W-X2. Alternatively, activation of these genes can be achieved by incubating somatic cell chromatin or nuclei with an extract prepared from Xenopus oocytes. This effect is not specific for 5S RNA genes as the transcription of other small RNAs (including pre-tRNA) is stimulated by the oocyte extract. The activating factor(s) is resistant to micrococcal nuclease, nondialyzable, heat labile and sensitive to trypsin; thus it is highly likely to be a protein or a group of proteins. Partial purification of the activating factor(s) has been achieved by ion exchange chromatography.
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978, Pubmed,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Butler,
Changes in chromatin folding in solution.
1980,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Ford,
Control of 5S RNA synthesis in Xenopus laevis.
1976,
Pubmed
,
Xenbase
Gottesfeld,
Organization of 5S genes in chromatin of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Gottesfeld,
Nonrandom alignment of nucleosomes on 5S RNA genes of X. laevis.
1980,
Pubmed
,
Xenbase
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase
Hörz,
Deoxyribonuclease II as a probe for chromatin structure. II. Mode of cleavage.
1980,
Pubmed
Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Korn,
Transcription of Xenopus 5S ribosomal RNA genes.
1982,
Pubmed
,
Xenbase
Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed
Ng,
Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
1979,
Pubmed
,
Xenbase
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Pelham,
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
1981,
Pubmed
,
Xenbase
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sanders,
Fractionation of nucleosomes by salt elution from micrococcal nuclease-digested nuclei.
1978,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Spadafora,
Rearrangement of chromatin structure induced by increasing ionic strength and temperature.
1979,
Pubmed
Sugden,
Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.
1973,
Pubmed
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Weinstock,
Intragenic DNA spacers interrupt the ovalbumin gene.
1978,
Pubmed
Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
