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Küntziger T
,
Gavet O
,
Manceau V
,
Sobel A
,
Bornens M
.
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Stathmin/Op 18 is a microtubule (MT) dynamics-regulating protein that has been shown to have both catastrophe-promoting and tubulin-sequestering activities. The level of stathmin/Op18 phosphorylation was proved both in vitro and in vivo to be important in modulating its MT-destabilizing activity. To understand the in vivo regulation of stathmin/Op18 activity, we investigated whether MT assembly itself could control phosphorylation of stathmin/Op18 and thus its MT-destabilizing activity. We found that MT nucleation by centrosomes from Xenopus sperm or somatic cells and MT assembly promoted by dimethyl sulfoxide or paclitaxel induced stathmin/Op18 hyperphosphorylation in Xenopus egg extracts, leading to new stathmin/Op18 isoforms phosphorylated on Ser 16. The MT-dependent phosphorylation of stathmin/Op18 took place in interphase extracts as well, and was also observed in somatic cells. We show that the MT-dependent phosphorylation of stathmin/Op18 on Ser 16 is mediated by an activity associated to the MTs, and that it is responsible for the stathmin/Op18 hyperphosphorylation reported to be induced by the addition of "mitotic chromatin." Our results suggest the existence of a positive feedback loop, which could represent a novel mechanism contributing to MT network control.
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997, Pubmed,
Xenbase
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997,
Pubmed
,
Xenbase
Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase
Beretta,
Multiple phosphorylation of stathmin. Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2.
1993,
Pubmed
Bornens,
Studying the composition and function of centrosomes in vertebrates.
1999,
Pubmed
Bornens,
[Method of isolation of the nuclear membrane of rat liver].
1968,
Pubmed
Brattsand,
Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38.
1994,
Pubmed
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Curmi,
The stathmin/tubulin interaction in vitro.
1997,
Pubmed
Gaglio,
NuMA is required for the organization of microtubules into aster-like mitotic arrays.
1995,
Pubmed
Gavet,
The stathmin phosphoprotein family: intracellular localization and effects on the microtubule network.
1998,
Pubmed
Geissler,
A novel protein complex promoting formation of functional alpha- and gamma-tubulin.
1998,
Pubmed
Gigant,
The 4 A X-ray structure of a tubulin:stathmin-like domain complex.
2000,
Pubmed
Gradin,
Regulation of microtubule dynamics by extracellular signals: cAMP-dependent protein kinase switches off the activity of oncoprotein 18 in intact cells.
1998,
Pubmed
Horwitz,
The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation.
1997,
Pubmed
Howell,
Decreasing oncoprotein 18/stathmin levels reduces microtubule catastrophes and increases microtubule polymer in vivo.
1999,
Pubmed
Howell,
Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin.
1999,
Pubmed
Jourdain,
Stathmin: a tubulin-sequestering protein which forms a ternary T2S complex with two tubulin molecules.
1997,
Pubmed
Kahana,
Beyond nuclear transport. Ran-GTP as a determinant of spindle assembly.
1999,
Pubmed
,
Xenbase
Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase
Koppel,
Developmental tissue expression and phylogenetic conservation of stathmin, a phosphoprotein associated with cell regulations.
1990,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Larsson,
Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis.
1997,
Pubmed
Larsson,
Op18/stathmin mediates multiple region-specific tubulin and microtubule-regulating activities.
1999,
Pubmed
Lawler,
Stathmin overexpression in 293 cells affects signal transduction and cell growth.
1998,
Pubmed
Luo,
Regulation of phosphoprotein p18 in leukemic cells. Cell cycle regulated phosphorylation by p34cdc2 kinase.
1994,
Pubmed
Marklund,
Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics.
1996,
Pubmed
Marklund,
Serine 16 of oncoprotein 18 is a major cytosolic target for the Ca2+/calmodulin-dependent kinase-Gr.
1994,
Pubmed
Maucuer,
Stathmin gene family: phylogenetic conservation and developmental regulation in Xenopus.
1993,
Pubmed
,
Xenbase
Melander Gradin,
Regulation of microtubule dynamics by Ca2+/calmodulin-dependent kinase IV/Gr-dependent phosphorylation of oncoprotein 18.
1997,
Pubmed
Morishima-Kawashima,
The pool of map kinase associated with microtubules is small but constitutively active.
1996,
Pubmed
Moudjou,
gamma-Tubulin in mammalian cells: the centrosomal and the cytosolic forms.
1996,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Ohta,
Ca2+/calmodulin-dependent protein kinase II: localization in the interphase nucleus and the mitotic apparatus of mammalian cells.
1990,
Pubmed
Ookata,
Association of p34cdc2/cyclin B complex with microtubules in starfish oocytes.
1993,
Pubmed
Pollister,
THE NUCLEOPROTAMINE OF TROUT SPERM.
1946,
Pubmed
Reszka,
Association of mitogen-activated protein kinase with the microtubule cytoskeleton.
1995,
Pubmed
Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase
Shapiro,
Activation of the MKK/ERK pathway during somatic cell mitosis: direct interactions of active ERK with kinetochores and regulation of the mitotic 3F3/2 phosphoantigen.
1998,
Pubmed
Shinohara-Gotoh,
Activation of microtubule-associated protein kinase by microtubule disruption in quiescent rat 3Y1 cells.
1991,
Pubmed
Sobel,
Intracellular substrates for extracellular signaling. Characterization of a ubiquitous, neuron-enriched phosphoprotein (stathmin).
1989,
Pubmed
Sobel,
Distinct patterns of cytoplasmic protein phosphorylation related to regulation of synthesis and release of prolactin by GH cells.
1983,
Pubmed
Sobel,
Stathmin: a relay phosphoprotein for multiple signal transduction?
1991,
Pubmed
Solomon,
Analyses of the cytoskeleton in Saccharomyces cerevisiae.
1991,
Pubmed
Sontag,
A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle.
1995,
Pubmed
Srivastava,
Involvement of microtubules in the regulation of Bcl2 phosphorylation and apoptosis through cyclic AMP-dependent protein kinase.
1998,
Pubmed
Steinmetz,
Op18/stathmin caps a kinked protofilament-like tubulin tetramer.
2000,
Pubmed
Strahler,
Cell cycle progression is associated with distinct patterns of phosphorylation of Op18.
1992,
Pubmed
Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase
Vega,
An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins.
1998,
Pubmed
le Gouvello,
Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.
1998,
Pubmed
