Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Sep 16;9419:10122-7. doi: 10.1073/pnas.94.19.10122.
Show Gene links
Show Anatomy links
Selection and nuclear immobilization of exportable RNAs.
Grimm C
,
Lund E
,
Dahlberg JE
.
???displayArticle.abstract???
The intracellular distribution of RNAs depends on interactions of cis-acting nuclear export elements or nuclear retention elements with trans-acting nuclear transport or retention factors. To learn about the relationship between export and retention, we isolated RNAs that are exported from nuclei of Xenopus laevis oocytes even when most RNA export is blocked by an inhibitor of Ran-dependent nucleocytoplasmic transport, the Matrix protein of vesicular stomatitis virus. Export of the selected RNAs is saturable and specific. When present in chimeric RNAs, the selected sequences acted like nuclear export elements in promoting efficient export of RNAs that otherwise are not exported; the pathway used for export of these chimeric RNAs is that used for the selected RNAs alone. However, these chimeric RNAs, unlike the selected RNAs, were not exported in the presence of Matrix protein; thus, the nonselected sequences can cause retention of the selected RNA sequences under conditions of impaired nucleocytoplasmic transport. We propose that most RNAs are transiently immobilized in the nucleus and that release of these RNAs is an essential and early step in export. Release correlates with functional Ran-dependent transport, and the lack of export of chimeric RNAs may result from interference with the Ran system.
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995, Pubmed,
Xenbase
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Cadwell,
Randomization of genes by PCR mutagenesis.
1992,
Pubmed
Chang,
Regulation by HIV Rev depends upon recognition of splice sites.
1989,
Pubmed
Cheng,
Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport.
1995,
Pubmed
Company,
Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes.
1991,
Pubmed
Dahlberg,
Coupling of nuclear RNA export and protein import in vertebrate cells.
1997,
Pubmed
Ernst,
A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.
1997,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Grimm,
In vivo selection of RNAs that localize in the nucleus.
1997,
Pubmed
,
Xenbase
Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed
Her,
Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus.
1997,
Pubmed
,
Xenbase
Izaurralde,
RNA export.
1995,
Pubmed
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Konarska,
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
1987,
Pubmed
Liang,
A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA.
1996,
Pubmed
Malim,
The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
1989,
Pubmed
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Moore,
The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.
1993,
Pubmed
,
Xenbase
Neuman de Vegvar,
Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
1990,
Pubmed
,
Xenbase
Ohno,
A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
1996,
Pubmed
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Richards,
Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export.
1997,
Pubmed
Saavedra,
Regulation of mRNA export in response to stress in Saccharomyces cerevisiae.
1996,
Pubmed
Simons,
The interactions with Ro60 and La differentially affect nuclear export of hY1 RNA.
1996,
Pubmed
,
Xenbase
Simons,
Analysis of the intracellular localization and assembly of Ro ribonucleoprotein particles by microinjection into Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Tang,
A cellular cofactor for the constitutive transport element of type D retrovirus.
1997,
Pubmed
Terns,
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.
1993,
Pubmed
,
Xenbase
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Terns,
A common maturation pathway for small nucleolar RNAs.
1995,
Pubmed
,
Xenbase
