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Nuclear transport of the U1 snRNP-specific protein U1A has been examined. U1A moves to the nucleus by an active process which is independent of interaction with U1 snRNA. Nuclear localization requires an unusually large sequence element situated between amino acids 94 and 204 of the protein. U1A transport is not unidirectional. The protein shuttles between nucleus and cytoplasm. At equilibrium, the concentration of the protein in the nucleus and cytoplasm is not, however, determined solely by transport rates, but can be perturbed by introducing RNA sequences that can specifically bind U1A in either the nuclear or cytoplasmic compartment. Thus, U1A represents a novel class of protein which shuttles between cytoplasm and nucleus and whose intracellular distribution can be altered by the number of free binding sites for the protein present in the cytoplasm or the nucleus.
Boelens,
Analysis of in vitro binding of U1-A protein mutants to U1 snRNA.
1991, Pubmed
Boelens,
Analysis of in vitro binding of U1-A protein mutants to U1 snRNA.
1991,
Pubmed
Borer,
Major nucleolar proteins shuttle between nucleus and cytoplasm.
1989,
Pubmed
,
Xenbase
Breeuwer,
Facilitated nuclear transport of histone H1 and other small nucleophilic proteins.
1990,
Pubmed
Bujard,
A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo.
1987,
Pubmed
Dabauvalle,
Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin.
1988,
Pubmed
,
Xenbase
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eliceiri,
Short-lived, small RNAs in the cytoplasm of HeLa cells.
1974,
Pubmed
Feeney,
Cytoplasmic assembly and nuclear accumulation of mature small nuclear ribonucleoprotein particles.
1989,
Pubmed
Feeney,
Nuclear exchange of the U1 and U2 snRNP-specific proteins.
1990,
Pubmed
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase
Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase
Fischer,
Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport.
1991,
Pubmed
,
Xenbase
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Gaillard,
Ethanol precipitation of DNA with linear polyacrylamide as carrier.
1990,
Pubmed
Garcia-Bustos,
Nuclear protein localization.
1991,
Pubmed
Goldfarb,
Nuclear transport.
1989,
Pubmed
Goldfarb,
Shuttling proteins go both ways.
1991,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hamm,
Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.
1990,
Pubmed
,
Xenbase
Heinrichs,
U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site.
1990,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Krohne,
The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.
1989,
Pubmed
,
Xenbase
Lamond,
The trimethyl-guanosine cap is a nuclear targeting signal for snRNPs.
1990,
Pubmed
Lanford,
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
1984,
Pubmed
Lehmeier,
Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5.
1990,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Lutz-Freyermuth,
Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA.
1990,
Pubmed
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Mandell,
Identification of two HSP70-related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope.
1990,
Pubmed
,
Xenbase
Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Michaud,
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.
1992,
Pubmed
,
Xenbase
Mount,
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
1983,
Pubmed
Nagai,
Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A.
1990,
Pubmed
Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase
Pan,
U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing.
1989,
Pubmed
,
Xenbase
Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase
Rihs,
Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen.
1989,
Pubmed
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase
Silver,
Mutations that alter both localization and production of a yeast nuclear protein.
1988,
Pubmed
Silver,
How proteins enter the nucleus.
1991,
Pubmed
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Zieve,
Newly synthesized small nuclear RNAs appear transiently in the cytoplasm.
1988,
Pubmed
