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We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb.
Boekhoff,
Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants.
1990, Pubmed
Boekhoff,
Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants.
1990,
Pubmed
Boekhoff,
Single odors differentially stimulate dual second messenger pathways in lobster olfactory receptor cells.
1994,
Pubmed
Buck,
A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
1991,
Pubmed
Caprio,
Electrophysiological evidence for acidic, basic, and neutral amino acid olfactory receptor sites in the catfish.
1984,
Pubmed
Edwards,
A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system.
1989,
Pubmed
Freitag,
Two classes of olfactory receptors in Xenopus laevis.
1995,
Pubmed
,
Xenbase
Friedrich,
Combinatorial and chemotopic odorant coding in the zebrafish olfactory bulb visualized by optical imaging.
1997,
Pubmed
Frings,
Current recording from sensory cilia of olfactory receptor cells in situ. I. The neuronal response to cyclic nucleotides.
1991,
Pubmed
Fuss,
Odorant feature detection: activity mapping of structure response relationships in the zebrafish olfactory bulb.
2001,
Pubmed
Gold,
Controversial issues in vertebrate olfactory transduction.
1999,
Pubmed
Iida,
Responses of Xenopus laevis water nose to water-soluble and volatile odorants.
1999,
Pubmed
,
Xenbase
Iida,
Responses to putative second messengers and odorants in water nose olfactory neurons of Xenopus laevis.
2000,
Pubmed
,
Xenbase
Kang,
In vivo responses of single olfactory receptor neurons of channel catfish to binary mixtures of amino acids.
1997,
Pubmed
Kang,
In vivo responses of single olfactory receptor neurons in the channel catfish, Ictalurus punctatus.
1995,
Pubmed
Kashiwayanagi,
Odor responses after complete desensitization of the cAMP-dependent pathway in turtle olfactory cells.
1995,
Pubmed
Michel,
Odor-evoked inhibition in primary olfactory receptor neurons.
1994,
Pubmed
Mombaerts,
Visualizing an olfactory sensory map.
1996,
Pubmed
Nezlin,
Structure of the olfactory bulb in tadpoles of Xenopus laevis.
2000,
Pubmed
,
Xenbase
Nikonov,
Electrophysiological evidence for a chemotopy of biologically relevant odors in the olfactory bulb of the channel catfish.
2001,
Pubmed
Reisert,
Responses to prolonged odour stimulation in frog olfactory receptor cells.
2001,
Pubmed
Ressler,
Information coding in the olfactory system: evidence for a stereotyped and highly organized epitope map in the olfactory bulb.
1994,
Pubmed
Restrepo,
Odor stimuli trigger influx of calcium into olfactory neurons of the channel catfish.
1990,
Pubmed
Schild,
Microcontrollers as inexpensive pulse generators and parallel processors in electrophysiological experiments.
1996,
Pubmed
Schild,
Transduction mechanisms in vertebrate olfactory receptor cells.
1998,
Pubmed
Sklar,
The odorant-sensitive adenylate cyclase of olfactory receptor cells. Differential stimulation by distinct classes of odorants.
1986,
Pubmed
Vogler,
Inhibitory and excitatory responses of olfactory receptor neurons of xenopus laevis tadpoles to stimulation with amino acids.
1999,
Pubmed
,
Xenbase
Zufall,
Amplification of odor-induced Ca(2+) transients by store-operated Ca(2+) release and its role in olfactory signal transduction.
2000,
Pubmed
Zufall,
From odor and pheromone transduction to the organization of the sense of smell.
2001,
Pubmed
