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Phorbol 12-myristate 13-acetate down-regulates Na,K-ATPase independent of its protein kinase C site: decrease in basolateral cell surface area.
Beron J
,
Forster I
,
Beguin P
,
Geering K
,
Verrey F
.
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The effect of protein kinase C (PKC) stimulation on the pump current (Ip) generated by the Na,K-ATPase was measured in A6 epithelia apically permeabilized with amphotericin B. Phorbol 12-myristate 13-acetate (PMA) produced a decrease in Ip carried by sodium pumps containing the endogenous Xenopus laevis or transfected Bufo marinus alpha 1 subunits (approximately 30% reduction within 25 min, maximum after 40 min) independent of the PKC phosphorylation site (T15A/S16A). In addition to this major effect of PMA, which was independent of the intracellular sodium concentration and was prevented by the PKC inhibitor bisindolylmaleimide GF 109203X (BIM), another BIM-resistant, PKC site-independent decrease was observed when the Ip was measured at low sodium concentrations (total reduction approximately 50% at 5 mM sodium). Using ouabain binding and cell surface biotinylation, stimulation of PKC was shown to reduce surface Na,K-ATPase by 14 to 20% within 25 min. The same treatment stimulated fluid phase endocytosis sevenfold and decreased by 16.5% the basolateral cell surface area measured by transepithelial capacitance measurements. In conclusion, PKC stimulation produces a decrease in sodium pump function which can be attributed, to a large extent, to a withdrawal of sodium pumps from the basolateral cell surface independent of their PKC site. This reduction of the number of sodium pumps is parallel to a decrease in basolateral membrane area.
Beguin,
Phosphorylation of the Na,K-ATPase alpha-subunit by protein kinase A and C in vitro and in intact cells. Identification of a novel motif for PKC-mediated phosphorylation.
1994, Pubmed,
Xenbase
Beguin,
Phosphorylation of the Na,K-ATPase alpha-subunit by protein kinase A and C in vitro and in intact cells. Identification of a novel motif for PKC-mediated phosphorylation.
1994,
Pubmed
,
Xenbase
Beron,
Aldosterone induces early activation and late accumulation of Na-K-ATPase at surface of A6 cells.
1994,
Pubmed
,
Xenbase
Beron,
Aldosterone modulates sodium kinetics of Na,K-ATPase containing an alpha 1 subunit in A6 kidney cell epithelia.
1995,
Pubmed
Bertorello,
Na+-K+-ATPase is an effector protein for protein kinase C in renal proximal tubule cells.
1989,
Pubmed
Bertorello,
Phosphorylation of the catalytic subunit of Na+,K(+)-ATPase inhibits the activity of the enzyme.
1991,
Pubmed
Chibalin,
Phosphorylation of Na,K-ATPase alpha-subunits in microsomes and in homogenates of Xenopus oocytes resulting from the stimulation of protein kinase A and protein kinase C.
1992,
Pubmed
,
Xenbase
Eaton,
Renal sodium channels: regulation and single channel properties.
1995,
Pubmed
Ewart,
Hormonal regulation of the Na(+)-K(+)-ATPase: mechanisms underlying rapid and sustained changes in pump activity.
1995,
Pubmed
Feschenko,
Structural basis for species-specific differences in the phosphorylation of Na,K-ATPase by protein kinase C.
1995,
Pubmed
Frindt,
Feedback regulation of Na channels in rat CCT. IV. Mediation by activation of protein kinase C.
1996,
Pubmed
Féraille,
Protein kinase C-dependent stimulation of Na(+)-K(+)-ATP epsilon in rat proximal convoluted tubules.
1995,
Pubmed
Gottardi,
Biotinylation and assessment of membrane polarity: caveats and methodological concerns.
1995,
Pubmed
Hays,
Effects of protein kinase C activation on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule.
1987,
Pubmed
Jaisser,
Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump.
1992,
Pubmed
,
Xenbase
Ling,
Effects of luminal Na+ on single Na+ channels in A6 cells, a regulatory role for protein kinase C.
1989,
Pubmed
Ling,
Inhibition of apical Na+ channels in rabbit cortical collecting tubules by basolateral prostaglandin E2 is modulated by protein kinase C.
1992,
Pubmed
Lowndes,
Kinetics of phosphorylation of Na+/K(+)-ATPase by protein kinase C.
1990,
Pubmed
Martiny-Baron,
Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976.
1993,
Pubmed
Middleton,
Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells.
1993,
Pubmed
Satoh,
Different mechanisms of renal Na-K-ATPase regulation by protein kinases in proximal and distal nephron.
1993,
Pubmed
Shahedi,
Protein kinase C activation causes inhibition of Na/K-ATPase activity in Madin-Darby canine kidney epithelial (MDCK) cells.
1992,
Pubmed
Takeda,
Inhibition of amiloride-sensitive apical Na+ conductance by acetylcholine in rabbit cortical collecting duct perfused in vitro.
1994,
Pubmed
Toullec,
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
1991,
Pubmed
Vasilets,
Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K(+)-ATPase in oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Verrey,
Corticosteroid regulation of renal Na,K-ATPase.
1996,
Pubmed
Verrey,
Polarized membrane movements in A6 kidney cells are regulated by aldosterone and vasopressin/vasotocin.
1993,
Pubmed
,
Xenbase
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Wilborn,
Differential expression of PKC isoforms in fresh and cultured rabbit CCD.
1996,
Pubmed
Wilkinson,
Protein kinase C: is its pivotal role in cellular activation over-stated?
1994,
Pubmed
Yanase,
Activators of protein kinase C inhibit sodium transport in A6 epithelia.
1986,
Pubmed
,
Xenbase
