Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes.
King BF
,
Townsend-Nicholson A
,
Wildman SS
,
Thomas T
,
Spyer KM
,
Burnstock G
.
???displayArticle.abstract???
Transcripts for P2X(2) and P2X(6) subunits are present in rat CNS and frequently colocalize in the same brainstem nuclei. When rat P2X(2) (rP2X(2)) and rat P2X(6) (rP2X(6)) receptors were expressed individually in Xenopus oocytes and studied under voltage-clamp conditions, only homomeric rP2X(2) receptors were fully functional and gave rise to large inward currents (2-3 microA) to extracellular ATP. Coexpression of rP2X(2) and rP2X(6) subunits in Xenopus oocytes resulted in a heteromeric rP2X(2/6) receptor, which showed a significantly different phenotype from the wild-type rP2X(2) receptor. Differences included reduction in agonist potencies and, in some cases (e.g., Ap(4)A), significant loss of agonist activity. ATP-evoked inward currents were biphasic at the heteromeric rP2X(2/6) receptor, particularly when Zn(2+) ions were present or extracellular pH was lowered. The pH range was narrower for H(+) enhancement of ATP responses at the heteromeric rP2X(2/6) receptor. Also, H(+) ions inhibited ATP responses at low pH levels (<pH 6.3). The pH-dependent blocking activity of suramin was changed at this heteromeric receptor, although the potentiating effect of Zn(2+) on ATP responses was unchanged. Thus, the rP2X(2/6) receptor is a functionally modified P2X(2)-like receptor with a distinct pattern of pH modulation of ATP activation and suramin blockade. Although homomeric P2X(6) receptors function poorly, the P2X(6) subunit can contribute to functional heteromeric P2X channels and may influence the phenotype of native P2X receptors in those cells in which it is expressed.
Arunlakshana,
Some quantitative uses of drug antagonists. 1958.
1997, Pubmed
Arunlakshana,
Some quantitative uses of drug antagonists. 1958.
1997,
Pubmed
Barnard,
International Union of Pharmacology. XV. Subtypes of gamma-aminobutyric acidA receptors: classification on the basis of subunit structure and receptor function.
1998,
Pubmed
Buell,
P2X receptors: an emerging channel family.
1996,
Pubmed
Burnstock,
The past, present and future of purine nucleotides as signalling molecules.
1997,
Pubmed
Collo,
Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels.
1996,
Pubmed
Comer,
Detection of weakly expressed genes in the rostral ventrolateral medulla of the rat using micropunch and reverse transcription-polymerase chain reaction techniques.
1997,
Pubmed
Ding,
Single channel properties of P2X2 purinoceptors.
1999,
Pubmed
,
Xenbase
Ding,
Ion permeation and block of P2X(2) purinoceptors: single channel recordings.
1999,
Pubmed
Haines,
Properties of the novel ATP-gated ionotropic receptor composed of the P2X(1) and P2X(5) isoforms.
1999,
Pubmed
Khakh,
Electrophysiological properties of P2X-purinoceptors in rat superior cervical, nodose and guinea-pig coeliac neurones.
1995,
Pubmed
Khakh,
Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds.
1999,
Pubmed
Kim,
Molecular assembly of the extracellular domain of P2X2, an ATP-gated ion channel.
1997,
Pubmed
King,
Effects of extracellular pH on agonism and antagonism at a recombinant P2X2 receptor.
1997,
Pubmed
,
Xenbase
King,
A novel P1 purinoceptor activates an outward K+ current in follicular oocytes of Xenopus laevis.
1996,
Pubmed
,
Xenbase
King,
Full sensitivity of P2X2 purinoceptor to ATP revealed by changing extracellular pH.
1996,
Pubmed
,
Xenbase
King,
P2 purinoceptor-activated inward currents in follicular oocytes of Xenopus laevis.
1996,
Pubmed
,
Xenbase
Lewis,
Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons.
1995,
Pubmed
Lê,
Functional and biochemical evidence for heteromeric ATP-gated channels composed of P2X1 and P2X5 subunits.
1999,
Pubmed
,
Xenbase
Lê,
Central P2X4 and P2X6 channel subunits coassemble into a novel heteromeric ATP receptor.
1998,
Pubmed
,
Xenbase
Nakamura,
P2Y1 purinergic receptors in sensory neurons: contribution to touch-induced impulse generation.
1996,
Pubmed
,
Xenbase
Nicke,
P2X1 and P2X3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels.
1998,
Pubmed
,
Xenbase
Pintor,
Presence of diadenosine polyphosphates--Ap4A and Ap5A--in rat brain synaptic terminals. Ca2+ dependent release evoked by 4-aminopyridine and veratridine.
1992,
Pubmed
Pintor,
Selectivity and activity of adenine dinucleotides at recombinant P2X2 and P2Y1 purinoceptors.
1996,
Pubmed
,
Xenbase
Radford,
Baculovirus expression provides direct evidence for heteromeric assembly of P2X2 and P2X3 receptors.
1997,
Pubmed
Soto,
Cloning and tissue distribution of a novel P2X receptor from rat brain.
1996,
Pubmed
,
Xenbase
Stoop,
Different sensitivities to pH of ATP-induced currents at four cloned P2X receptors.
1997,
Pubmed
Stoop,
Fading and rebound of P2X2 currents at millimolar ATP concentrations caused by low pH.
1998,
Pubmed
Thomas,
Central CO2 chemoreception: a mechanism involving P2 purinoceptors localized in the ventrolateral medulla of the anaesthetized rat.
1999,
Pubmed
Thomas,
ATP as a mediator of mammalian central CO2 chemoreception.
2000,
Pubmed
Torres,
Hetero-oligomeric assembly of P2X receptor subunits. Specificities exist with regard to possible partners.
1999,
Pubmed
Torres,
Co-expression of P2X1 and P2X5 receptor subunits reveals a novel ATP-gated ion channel.
1998,
Pubmed
Townsend-Nicholson,
Molecular cloning, functional characterization and possible cooperativity between the murine P2X4 and P2X4a receptors.
1999,
Pubmed
,
Xenbase
Virginio,
Effects of divalent cations, protons and calmidazolium at the rat P2X7 receptor.
1997,
Pubmed
Virginio,
Pore dilation of neuronal P2X receptor channels.
1999,
Pubmed
Wildman,
Potentiation of ATP-responses at a recombinant P2x2 receptor by neurotransmitters and related substances.
1997,
Pubmed
,
Xenbase
Wildman,
Selectivity of diadenosine polyphosphates for rat P2X receptor subunits.
1999,
Pubmed
,
Xenbase
Wildman,
Modulatory activity of extracellular H+ and Zn2+ on ATP-responses at rP2X1 and rP2X3 receptors.
1999,
Pubmed
,
Xenbase
Wildman,
Modulation of ATP-responses at recombinant rP2X4 receptors by extracellular pH and zinc.
1999,
Pubmed
,
Xenbase
Wildman,
Zn2+ modulation of ATP-responses at recombinant P2X2 receptors and its dependence on extracellular pH.
1998,
Pubmed
,
Xenbase
Worthington,
Localisation of P2X receptors in human salivary gland epithelial cells and human embryonic kidney cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting and immunofluorescence.
1999,
Pubmed
Ziganshin,
Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown.
1995,
Pubmed
,
Xenbase
