Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Single amino acid substitution affects desensitization of the 5-hydroxytryptamine type 3 receptor expressed in Xenopus oocytes.
Yakel JL
,
Lagrutta A
,
Adelman JP
,
North RA
.
???displayArticle.abstract???
5-Hydroxytryptamine type 3 receptors were expressed in Xenopus oocytes from a cloned cDNA. The peak inward current evoked by 5-hydroxytryptamine (30 microM) was linearly related to the holding potential (-100 to +20 mV) and reversed near 0 mV. The inward current (at -60 mV) declined during the continued presence of 5-hydroxytryptamine with a half-time of about 2 s; this desensitization was 20 times slower in calcium-free solution. Desensitization was markedly different in channels in which Leu286 was changed by site-directed mutagenesis; this residue is thought to lie near the middle of the M2 segment. Desensitization was faster with Phe, Tyr, or Ala in this position and slower with Thr. Phe and Thr substitutions in the equivalent position of the nicotinic acetylcholine receptor have similar effects on desensitization, suggesting that the underlying conformational change might be common to ligand-gated channels.
Bertrand,
Unconventional pharmacology of a neuronal nicotinic receptor mutated in the channel domain.
1992, Pubmed,
Xenbase
Bertrand,
Unconventional pharmacology of a neuronal nicotinic receptor mutated in the channel domain.
1992,
Pubmed
,
Xenbase
Bettler,
Cloning of a novel glutamate receptor subunit, GluR5: expression in the nervous system during development.
1990,
Pubmed
,
Xenbase
Christie,
Expression of a cloned rat brain potassium channel in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Egebjerg,
Cloning of a cDNA for a glutamate receptor subunit activated by kainate but not AMPA.
1991,
Pubmed
,
Xenbase
Galzi,
Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic.
1992,
Pubmed
,
Xenbase
Higashi,
5-Hydroxytryptamine receptors of visceral primary afferent neurones on rabbit nodose ganglia.
1982,
Pubmed
Hollmann,
Cloning by functional expression of a member of the glutamate receptor family.
1989,
Pubmed
,
Xenbase
Huganir,
Regulation of neurotransmitter receptor desensitization by protein phosphorylation.
1990,
Pubmed
Huganir,
Phosphorylation of the nicotinic acetylcholine receptor regulates its rate of desensitization.
,
Pubmed
Isaacson,
Aniracetam reduces glutamate receptor desensitization and slows the decay of fast excitatory synaptic currents in the hippocampus.
1991,
Pubmed
,
Xenbase
Keinänen,
A family of AMPA-selective glutamate receptors.
1990,
Pubmed
Kilpatrick,
[3H] GR67330, a very high affinity ligand for 5-HT3 receptors.
1990,
Pubmed
Kutsuwada,
Molecular diversity of the NMDA receptor channel.
1992,
Pubmed
,
Xenbase
Lambert,
The properties of 5-HT3 receptors in clonal cell lines studied by patch-clamp techniques.
1989,
Pubmed
Maricq,
Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel.
1991,
Pubmed
,
Xenbase
Meguro,
Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs.
1992,
Pubmed
,
Xenbase
Moriyoshi,
Molecular cloning and characterization of the rat NMDA receptor.
1991,
Pubmed
,
Xenbase
Neijt,
Pharmacological characterization of serotonin 5-HT3 receptor-mediated electrical response in cultured mouse neuroblastoma cells.
1988,
Pubmed
Neijt,
Kinetics of the membrane current mediated by serotonin 5-HT3 receptors in cultured mouse neuroblastoma cells.
1989,
Pubmed
Peters,
Divalent cations modulate 5-HT3 receptor-induced currents in N1E-115 neuroblastoma cells.
1988,
Pubmed
Revah,
Mutations in the channel domain alter desensitization of a neuronal nicotinic receptor.
1991,
Pubmed
,
Xenbase
Robertson,
Properties of 5-hydroxytryptamine3 receptor-gated currents in adult rat dorsal root ganglion neurones.
1991,
Pubmed
Schoepfer,
Brain alpha-bungarotoxin binding protein cDNAs and MAbs reveal subtypes of this branch of the ligand-gated ion channel gene superfamily.
1990,
Pubmed
Shao,
Differentiation of NG108-15 cells alters channel conductance and desensitization kinetics of the 5-HT3 receptor.
1991,
Pubmed
Trussell,
Glutamate receptor desensitization and its role in synaptic transmission.
1989,
Pubmed
Unwin,
The structure of ion channels in membranes of excitable cells.
1989,
Pubmed
Vyklicky,
Modulation of excitatory synaptic transmission by drugs that reduce desensitization at AMPA/kainate receptors.
1991,
Pubmed
Yakel,
5-HT3 receptors mediate rapid responses in cultured hippocampus and a clonal cell line.
1988,
Pubmed
Yakel,
The selectivity of the channel coupled to the 5-HT3 receptor.
1990,
Pubmed
Yakel,
Activation and desensitization of the 5-HT3 receptor in a rat glioma x mouse neuroblastoma hybrid cell.
1991,
Pubmed
Yang,
Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells.
1990,
Pubmed
Yang,
5-HT3 receptor channels in dissociated rat superior cervical ganglion neurons.
1992,
Pubmed
