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Single-channel dose-response studies in single, cell-attached patches.
Auerbach A
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A method for carrying out dose-response studies of ion channel currents in cell-attached patches has been devised. Patch pipettes are filled at the tip with a solution containing one concentration of ligand and then backfilled with another. The concentration of ligand at the membrane is described as a function of time by the equation for diffusion in a cone, allowing response vs. time data to be transformed into a dose-response curve. For Xenopus myocyte cholinergic receptors, examples of the use of this method are given for several concentration-dependent reactions including blockade by the local anesthetic QX-222, activation by acetylcholine, and modulation of current amplitude by sodium ions. Several methods of analyzing the nonstationary channel kinetics are presented, including a pseudo-stationary approach that uses interval likelihood maximization.
Auerbach,
Activation of the primary kinetic modes of large- and small-conductance cholinergic ion channels in Xenopus myocytes.
1987, Pubmed,
Xenbase
Auerbach,
Activation of the primary kinetic modes of large- and small-conductance cholinergic ion channels in Xenopus myocytes.
1987,
Pubmed
,
Xenbase
Auerbach,
Heterogeneous kinetic properties of acetylcholine receptor channels in Xenopus myocytes.
1986,
Pubmed
,
Xenbase
Chay,
Kinetic modeling for the channel gating process from single channel patch clamp data.
1988,
Pubmed
Covarrubias,
Excision of membrane patches reduces the mean open time of nicotinic acetylcholine receptors.
1990,
Pubmed
Cull-Candy,
Single glutamate-activated channels recorded from locust muscle fibres with perfused patch-clamp electrodes.
1981,
Pubmed
Dionne,
Characterization of drug iontophoresis with a fast microassay technique.
1976,
Pubmed
Horn,
Muscarinic activation of ionic currents measured by a new whole-cell recording method.
1988,
Pubmed
Horn,
Statistical properties of single sodium channels.
1984,
Pubmed
Horn,
Estimating kinetic constants from single channel data.
1983,
Pubmed
Horn,
Single channel currents from excised patches of muscle membrane.
1980,
Pubmed
Liu,
Opening rate of acetylcholine receptor channels.
1991,
Pubmed
Mathias,
Limitations of the whole cell patch clamp technique in the control of intracellular concentrations.
1990,
Pubmed
Neher,
Local anaesthetics transiently block currents through single acetylcholine-receptor channels.
1978,
Pubmed
Pusch,
Rates of diffusional exchange between small cells and a measuring patch pipette.
1988,
Pubmed
Roux,
A general solution to the time interval omission problem applied to single channel analysis.
1985,
Pubmed
Ruknudin,
The ultrastructure of patch-clamped membranes: a study using high voltage electron microscopy.
1991,
Pubmed
,
Xenbase
Sachs,
The automated analysis of data from single ionic channels.
1982,
Pubmed
Sakmann,
Single acetylcholine-activated channels show burst-kinetics in presence of desensitizing concentrations of agonist.
1980,
Pubmed
Tang,
Perfusing pipettes.
1990,
Pubmed
