Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-44333
Genesis 2012 Mar 01;503:316-24. doi: 10.1002/dvg.20822.
Show Gene links Show Anatomy links

Characterization of a novel Xenopus tropicalis cell line as a model for in vitro studies.

Sinzelle L , Thuret R , Hwang HY , Herszberg B , Paillard E , Bronchain OJ , Stemple DL , Dhorne-Pollet S , Pollet N .


???displayArticle.abstract???
Cell lines are useful tools to facilitate in vitro studies of many biological and molecular processes. We describe a new permanent fibroblast-type cell line obtained from disaggregated Xenopus tropicalis limb bud. The cell line population doubling time was ~24 h. Its karyotype was genetically stable with a chromosome number of 2n = 21 and a chromosome 10 trisomy. These cells could be readily transfected and expressed transgenes faithfully. We obtained stable transformants using transposon-based gene transfer technology. These cells responded to thyroid hormone and thus can provide a complementary research tool to study thyroid hormone signaling events. In conclusion, this cell line baptized "Speedy" should prove useful to couple in vitro and in vivo biological studies in the X. tropicalis frog model.

???displayArticle.pubmedLink??? 22083648
???displayArticle.pmcLink??? PMC3503257
???displayArticle.link??? Genesis


Species referenced: Xenopus tropicalis
Genes referenced: epb41 ezh1 ezh2 fbxl7 gemin5 mast3 mat1a naif1 odc1 olig3 rpl8 spdya stat4 tbx2 thibz thra thrb trhd znf423


???attribute.lit??? ???displayArticles.show???
References [+] :
Anizet, Characterization of a new cell line, XL2, obtained from Xenopus laevis and determination of optimal culture conditions. 1981, Pubmed, Xenbase