Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Muscle Res Cell Motil
1989 Jun 01;103:221-8. doi: 10.1007/bf01739812.
Show Gene links
Show Anatomy links
Maximum rate of oxygen consumption and quantitative histochemistry of succinate dehydrogenase in single muscle fibres of Xenopus laevis.
van der Laarse WJ
,
Diegenbach PC
,
Elzinga G
.
???displayArticle.abstract???
Three different types of single living muscle fibre were dissected from the iliofibularismuscle of Xenopus laevis. The fibres were mounted in a glass chamber and their rate of oxygen consumption was determined as a function of twitch frequency at 20 degrees C. The rate of oxygen consumption increased with twitch frequency until it levelled off and reached a maximum. The maximum rate of oxygen consumption varied between fibres (0.019 to 0.161 nmol O2 s-1 mm-3) and was reached at different twitch frequencies (less than 0.2 to 5.7 stimuli s-1). After the determination of the maximum rate of oxygen consumption, the succinate dehydrogenase activity in cross sections of the fibre was determined by means of a quantitative histochemical method. A proportional relationship between the maximum rate of oxygen consumption and the succinate dehydrogenase activity was found. The maximum rate of oxygen consumption and the succinate dehydrogenase activity are also proportional to the volume density of mitochondria in the three fibre types reported by Smith and Ovalle (1973; J. Anat., Lond. 116, 1-24). It is concluded that quantitative histochemistry of succinate dehydrogenase reliably predicts the maximum rate of oxygen consumption of muscle fibres in Xenopus laevis and that the maximum rate of oxygen consumption of single muscle fibres is determined by the volume density of mitochondria.
Altman,
Quantitative microscopy of enzyme reactions in tissue sections.
1977, Pubmed
Altman,
Quantitative microscopy of enzyme reactions in tissue sections.
1977,
Pubmed
Araki,
Topographic estimations by component spectroanalysis of two formazans of nitroblue tetrazolium in tissue sections.
1987,
Pubmed
BLINKS,
INFLUENCE OF OSMOTIC STRENGTH ON CROSS-SECTION AND VOLUME OF ISOLATED SINGLE MUSCLE FIBRES.
1965,
Pubmed
Butcher,
Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry.
1972,
Pubmed
Crow,
Chemical energetics of slow- and fast-twitch muscles of the mouse.
1982,
Pubmed
Edman,
Maximum velocity of shortening related to myosin isoform composition in frog skeletal muscle fibres.
1988,
Pubmed
Elzinga,
Oxygen consumption of single muscle fibres of Rana temporaria and Xenopus laevis at 20 degrees C.
1988,
Pubmed
,
Xenbase
Holloszy,
Adaptations of skeletal muscle to endurance exercise and their metabolic consequences.
1984,
Pubmed
Hoppeler,
Relationship between mitochondria and oxygen consumption in isolated cat muscles.
1987,
Pubmed
Kugelberg,
Transmission and contraction fatigue of rat motor units in relation to succinate dehydrogenase activity of motor unit fibres.
1979,
Pubmed
Lännergren,
Myosin isoenzymes in single muscle fibres of Xenopus laevis: analysis of five different functional types.
1984,
Pubmed
,
Xenbase
PETTE,
Comparable and specific proportions in the mitochondrial enzyme activity pattern.
1962,
Pubmed
Pool,
Quantitative succinate-dehydrogenase histochemistry. I. A Methodological study on mammalian and fish muscle.
1979,
Pubmed
Reichmann,
Biochemical and ultrastructural changes of skeletal muscle mitochondria after chronic electrical stimulation in rabbits.
1985,
Pubmed
Sieck,
SDH activity and cross-sectional area of muscle fibers in cat diaphragm.
1986,
Pubmed
Smith,
Varieties of fast and slow extrafusal muscle fibres in amphibian hind limb muscles.
1973,
Pubmed
,
Xenbase
Swatland,
The radial distribution of succinate dehydrogenase activity in porcine muscle fibres.
1984,
Pubmed
Van Noorden,
Cytophotometry of glucose-6-phosphate dehydrogenase activity in individual cells.
1983,
Pubmed
Van der Laarse,
Method of quenching of muscle fibres affects apparent succinate dehydrogenase activity.
1988,
Pubmed
,
Xenbase
van Noorden,
Quantitative aspects of the cytochemical demonstration of glucose-6-phosphate dehydrogenase with tetrazolium salts studied in a model system of polyacrylamide films.
1981,
Pubmed
van der Laarse,
Quantitative histochemistry of three mouse hind-limb muscles: the relationship between calcium-stimulated myofibrillar ATPase and succinate dehydrogenase activities.
1984,
Pubmed
van der Laarse,
Calcium-stimulated myofibrillar ATPase activity correlates with shortening velocity of muscle fibres in Xenopus laevis.
1986,
Pubmed
,
Xenbase
