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Ion channel activity of influenza A virus M2 protein: characterization of the amantadine block.
Wang C
,
Takeuchi K
,
Pinto LH
,
Lamb RA
.
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The influenza A virus M2 integral membrane protein has ion channel activity which can be blocked by the antiviral drug amantadine. The M2 protein transmembrane domain is highly conserved in amino acid sequence for all the human, swine, equine, and avian strains of influenza A virus, and thus, known amino acid differences could lead to altered properties of the M2 ion channel. We have expressed in oocytes of Xenopus laevis the M2 protein of human influenza virus A/Udorn/72 and the avian virus A/chicken/Germany/34 (fowl plague virus, Rostock) and derivatives of the Rostock ion channel altered in the presumed pore region. The pH of activation of the M2 ion channels and amantadine block of the M2 ion channels were investigated. The channels were found to be activated by pH in a similar manner but differed in their apparent Kis for amantadine block.
ARMSTRONG,
ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE.
1965, Pubmed
ARMSTRONG,
ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE.
1965,
Pubmed
Belshe,
Genetic basis of resistance to rimantadine emerging during treatment of influenza virus infection.
1988,
Pubmed
Bukrinskaya,
Influenza virus uncoating in infected cells and effect of rimantadine.
1982,
Pubmed
Ciampor,
Evidence that the amantadine-induced, M2-mediated conversion of influenza A virus hemagglutinin to the low pH conformation occurs in an acidic trans Golgi compartment.
1992,
Pubmed
Ciampor,
Regulation of pH by the M2 protein of influenza A viruses.
1992,
Pubmed
DeLean,
Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.
1978,
Pubmed
Duff,
The transmembrane domain of influenza A M2 protein forms amantadine-sensitive proton channels in planar lipid bilayers.
1992,
Pubmed
Epand,
Effects of viral chemotherapeutic agents on membrane properties. Studies of cyclosporin A, benzyloxycarbonyl-D-Phe-L-Phe-Gly and amantadine.
1987,
Pubmed
Grambas,
Maturation of influenza A virus hemagglutinin--estimates of the pH encountered during transport and its regulation by the M2 protein.
1992,
Pubmed
Grambas,
Influence of amantadine resistance mutations on the pH regulatory function of the M2 protein of influenza A viruses.
1992,
Pubmed
Hay,
The molecular basis of the specific anti-influenza action of amantadine.
1985,
Pubmed
Hayden,
Emergence and transmission of influenza A viruses resistant to amantadine and rimantadine.
1992,
Pubmed
Helenius,
Unpacking the incoming influenza virus.
1992,
Pubmed
Higuchi,
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
1988,
Pubmed
Hille,
The selective inhibition of delayed potassium currents in nerve by tetraethylammonium ion.
1967,
Pubmed
Holsinger,
Influenza virus M2 integral membrane protein is a homotetramer stabilized by formation of disulfide bonds.
1991,
Pubmed
Ito,
Evolutionary analysis of the influenza A virus M gene with comparison of the M1 and M2 proteins.
1991,
Pubmed
Kato,
Inhibition of uncoating of fowl plague virus by l-adamantanamine hydrochloride.
1969,
Pubmed
Lamb,
Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.
1981,
Pubmed
Lamb,
Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription.
1976,
Pubmed
Lamb,
Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface.
1985,
Pubmed
Lamb,
Identification of a second protein (M2) encoded by RNA segment 7 of influenza virus.
1981,
Pubmed
Lamb,
Conservation of the influenza virus membrane protein (M1) amino acid sequence and an open reading frame of RNA segment 7 encoding a second protein (M2) in H1N1 and H3N2 strains.
1981,
Pubmed
Marsh,
Keeping the viral coat on.
1992,
Pubmed
Martin,
Nuclear transport of influenza virus ribonucleoproteins: the viral matrix protein (M1) promotes export and inhibits import.
1991,
Pubmed
Matsuda,
Open-state substructure of inwardly rectifying potassium channels revealed by magnesium block in guinea-pig heart cells.
1988,
Pubmed
McCauley,
Nucleotide sequence of fowl plague virus RNA segment 7.
1982,
Pubmed
Neher,
Local anaesthetics transiently block currents through single acetylcholine-receptor channels.
1978,
Pubmed
Pinto,
Influenza virus M2 protein has ion channel activity.
1992,
Pubmed
,
Xenbase
Sansom,
Influenza virus M2 protein: a molecular modelling study of the ion channel.
1993,
Pubmed
Skehel,
On the mechanism of inhibition of influenza virus replication by amantadine hydrochloride.
1978,
Pubmed
Skehel,
Influenza virus. Amantadine blocks the channel.
1992,
Pubmed
Stanfield,
Tetraethylammonium ions and the potassium permeability of excitable cells.
1983,
Pubmed
Steinhauer,
Amantadine selection of a mutant influenza virus containing an acid-stable hemagglutinin glycoprotein: evidence for virus-specific regulation of the pH of glycoprotein transport vesicles.
1991,
Pubmed
Sugrue,
Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel.
1991,
Pubmed
Zebedee,
Nucleotide sequences of influenza A virus RNA segment 7: a comparison of five isolates.
1989,
Pubmed
Zebedee,
Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions.
1988,
Pubmed
Zebedee,
Characterization of the influenza virus M2 integral membrane protein and expression at the infected-cell surface from cloned cDNA.
1985,
Pubmed
