Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-24227
Mol Biol Cell 1992 Jan 01;31:73-84. doi: 10.1091/mbc.3.1.73.
Show Gene links Show Anatomy links

cdc25+ encodes a protein phosphatase that dephosphorylates p34cdc2.

Lee MS , Ogg S , Xu M , Parker LL , Donoghue DJ , Maller JL , Piwnica-Worms H .


???displayArticle.abstract???
To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion protein (p80GST-cdc25) and was judged to be functional by several criteria: recombinant p80GST-cdc25 induced meiotic maturation of Xenopus oocytes in the presence of cycloheximide; p80GST-cdc25 activated histone H1 kinase activity upon addition to extracts prepared from Xenopus oocytes; p80GST-cdc25 activated p34cdc2/cyclin B complexes (prematuration promoting factor) in immune complex kinase assays performed in vitro; p80GST-cdc25 stimulated the tyrosine dephosphorylation of p34cdc2/cyclin complexes isolated from Xenopus oocyte extracts as well as from overproducing insect cells; and p80GST-cdc25 hydrolyzed p-nitrophenylphosphate. In addition, deletion analysis defined a functional domain residing within the carboxy-terminus of the cdc25Hs protein. Taken together, these results suggest that the cdc25Hs protein is itself a phosphatase and that it may function directly in the tyrosine dephosphorylation and activation of p34cdc2 at the G2 to M transition.

???displayArticle.pubmedLink??? 1312880
???displayArticle.pmcLink??? PMC275503
???displayArticle.link??? Mol Biol Cell
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: ccnb1.2 cdk1 rasgrf1

References [+] :
Beach, Functionally homologous cell cycle control genes in budding and fission yeast. 1982, Pubmed