Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
1992 Jan 01;31:73-84. doi: 10.1091/mbc.3.1.73.
Show Gene links
Show Anatomy links
cdc25+ encodes a protein phosphatase that dephosphorylates p34cdc2.
Lee MS
,
Ogg S
,
Xu M
,
Parker LL
,
Donoghue DJ
,
Maller JL
,
Piwnica-Worms H
.
???displayArticle.abstract???
To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion protein (p80GST-cdc25) and was judged to be functional by several criteria: recombinant p80GST-cdc25 induced meiotic maturation of Xenopus oocytes in the presence of cycloheximide; p80GST-cdc25 activated histone H1 kinase activity upon addition to extracts prepared from Xenopus oocytes; p80GST-cdc25 activated p34cdc2/cyclin B complexes (prematuration promoting factor) in immune complex kinase assays performed in vitro; p80GST-cdc25 stimulated the tyrosine dephosphorylation of p34cdc2/cyclin complexes isolated from Xenopus oocyte extracts as well as from overproducing insect cells; and p80GST-cdc25 hydrolyzed p-nitrophenylphosphate. In addition, deletion analysis defined a functional domain residing within the carboxy-terminus of the cdc25Hs protein. Taken together, these results suggest that the cdc25Hs protein is itself a phosphatase and that it may function directly in the tyrosine dephosphorylation and activation of p34cdc2 at the G2 to M transition.
Beach,
Functionally homologous cell cycle control genes in budding and fission yeast.
1982, Pubmed
Beach,
Functionally homologous cell cycle control genes in budding and fission yeast.
1982,
Pubmed
Blow,
A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts.
1990,
Pubmed
,
Xenbase
Booher,
The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization.
1989,
Pubmed
Brizuela,
p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
1987,
Pubmed
Chernoff,
Cloning of a cDNA for a major human protein-tyrosine-phosphatase.
1990,
Pubmed
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Draetta,
Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate.
,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Edgar,
Genetic control of cell division patterns in the Drosophila embryo.
1989,
Pubmed
Furukawa,
cdc2 gene expression at the G1 to S transition in human T lymphocytes.
1990,
Pubmed
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gould,
Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase.
1990,
Pubmed
Guan,
A Tyr/Ser protein phosphatase encoded by vaccinia virus.
1991,
Pubmed
Jimenez,
Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string.
1990,
Pubmed
Kaelin,
Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.
1991,
Pubmed
Krek,
Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.
1991,
Pubmed
Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed
,
Xenbase
Labbe,
Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lewin,
Driving the cell cycle: M phase kinase, its partners, and substrates.
1990,
Pubmed
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase
Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed
Meijer,
Cyclin B targets p34cdc2 for tyrosine phosphorylation.
1991,
Pubmed
,
Xenbase
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Moreno,
Regulation of p34cdc2 protein kinase during mitosis.
1989,
Pubmed
Moreno,
Clues to action of cdc25 protein.
1991,
Pubmed
Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Nurse,
Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast.
1981,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Parker,
Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner.
1991,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Pines,
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
1990,
Pubmed
Reed,
The selection of S. cerevisiae mutants defective in the start event of cell division.
1980,
Pubmed
Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase
Russell,
Conservation of mitotic controls in fission and budding yeasts.
1989,
Pubmed
Russell,
Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
1987,
Pubmed
Russell,
cdc25+ functions as an inducer in the mitotic control of fission yeast.
1986,
Pubmed
Sadhu,
Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
1990,
Pubmed
Simanis,
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
1986,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
,
Xenbase