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???displayArticle.abstract??? Xklp1 is a chromosome-associated kinesin required for Xenopus early embryonic cell division. Function blocking experiments in Xenopus egg extracts suggested that it is required for spindle assembly. We have reinvestigated Xklp1 function(s) by monitoring spindle assembly and microtubule behavior under a range of Xklp1 concentrations in egg extracts. We found that in the absence of Xklp1, bipolar spindles form with a reduced efficiency and display abnormalities associated with an increased microtubule mass. Likewise, centrosomal asters assembled in Xklp1-depleted extract show an increased microtubule mass. Conversely, addition of recombinant Xklp1 to the extract reduces the microtubule mass associated with spindles and asters. Our data suggest that Xklp1 affects microtubule polymerization during M-phase. We propose that these attributes, combined with Xklp1 plus-end directed motility, contribute to the assembly of a functional bipolar spindle.
Antonio,
Xkid, a chromokinesin required for chromosome alignment on the metaphase plate.
2000, Pubmed,
Xenbase
Antonio,
Xkid, a chromokinesin required for chromosome alignment on the metaphase plate.
2000,
Pubmed
,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Bornens,
Studying the composition and function of centrosomes in vertebrates.
1999,
Pubmed
Bringmann,
A kinesin-like motor inhibits microtubule dynamic instability.
2004,
Pubmed
,
Xenbase
Brust-Mascher,
Model for anaphase B: role of three mitotic motors in a switch from poleward flux to spindle elongation.
2004,
Pubmed
Budde,
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Carpenter,
Distributive segregation: motors in the polar wind?
1991,
Pubmed
Funabiki,
The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement.
2000,
Pubmed
,
Xenbase
Gruss,
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.
2002,
Pubmed
,
Xenbase
Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Heald,
Motor function in the mitotic spindle.
2000,
Pubmed
Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase
Kurasawa,
Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation.
2004,
Pubmed
Kwon,
The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility.
2004,
Pubmed
Lawrence,
A standardized kinesin nomenclature.
2004,
Pubmed
Mazumdar,
Human chromokinesin KIF4A functions in chromosome condensation and segregation.
2004,
Pubmed
Mitchison,
Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles.
2005,
Pubmed
,
Xenbase
Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase
Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase
Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase
Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Vernos,
Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning.
1995,
Pubmed
,
Xenbase
Walczak,
XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Walczak,
A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity.
,
Pubmed
,
Xenbase
Wittmann,
The spindle: a dynamic assembly of microtubules and motors.
2001,
Pubmed
Zhu,
Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis.
2005,
Pubmed
