Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1991 Oct 01;8819:8397-400.
Show Gene links
Show Anatomy links
Coexpression of alpha 1 with putative beta 3 subunits results in functional Na+/K+ pumps in Xenopus oocytes.
Horisberger JD
,
Jaunin P
,
Good PJ
,
Rossier BC
,
Geering K
.
???displayArticle.abstract???
The active Na+/K+ pump is composed of an alpha and a beta subunit. Until now, three putative isoforms of the beta subunit have been identified that share sequence similarity. We have expressed the beta 1 and beta 3 isoforms of Xenopus laevis Na+/K(+)-ATPase in Xenopus oocytes to compare functional properties of the Na+/K+ pump, including either of these two isoforms. Na+/K+ pump current, estimated as K(+)-induced outward current in voltage-clamped oocytes, was doubled by coexpression of alpha 1 subunits with either isoform of the beta subunit compared to expression of alpha 1 subunits alone. The kinetics of activation by external K+ and the voltage dependence of the electrogenic activity of the Na+/K+ pump were similar with both beta isoforms, indicating that both beta 1 and beta 3 isoforms can support expression at the oocyte surface of an active Na+/K+ pump with similar functional properties.
Ackermann,
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.
1990, Pubmed,
Xenbase
Ackermann,
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.
1990,
Pubmed
,
Xenbase
Apell,
Electrogenic properties of the Na,K pump.
1989,
Pubmed
Gadsby,
Steady-state current-voltage relationship of the Na/K pump in guinea pig ventricular myocytes.
1989,
Pubmed
Geering,
A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Geering,
Subunit assembly and functional maturation of Na,K-ATPase.
1990,
Pubmed
Gloor,
The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase.
1990,
Pubmed
Good,
A nervous system-specific isotype of the beta subunit of Na+,K(+)-ATPase expressed during early development of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Good,
Both VP2 and VP3 are synthesized from each of the alternative spliced late 19S RNA species of simian virus 40.
1988,
Pubmed
Horisberger,
Na-K pump current in the Amphiuma collecting tubule.
1989,
Pubmed
Horisberger,
Structure-function relationship of Na,K-ATPase.
1991,
Pubmed
Latorre,
Conduction and selectivity in potassium channels.
1983,
Pubmed
Läuger,
Dynamics of ion transport systems in membranes.
1987,
Pubmed
Martin-Vasallo,
Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression.
1989,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Noguchi,
Expression of functional (Na+ + K+)-ATPase from cloned cDNAs.
1987,
Pubmed
,
Xenbase
Rakowski,
A negative slope in the current-voltage relationship of the Na+/K+ pump in Xenopus oocytes produced by reduction of external [K+].
1991,
Pubmed
,
Xenbase
Rakowski,
Voltage dependence of Na/K pump current in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Richter,
Gene expression in the embryonic nervous system of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Schwarz,
Characteristics of the Na+/K+-ATPase from Torpedo californica expressed in Xenopus oocytes: a combination of tracer flux measurements with electrophysiological measurements.
1988,
Pubmed
,
Xenbase
Shyjan,
The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity.
1990,
Pubmed
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
