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XB-ART-48413
Dev Biol 2014 Feb 15;3862:461-72. doi: 10.1016/j.ydbio.2013.12.010.
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Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.

Plouhinec JL , Roche DD , Pegoraro C , Figueiredo AL , Maczkowiak F , Brunet LJ , Milet C , Vert JP , Pollet N , Harland RM , Monsoro-Burq AH .


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Neural crest development is orchestrated by a complex and still poorly understood gene regulatory network. Premigratory neural crest is induced at the lateral border of the neural plate by the combined action of signaling molecules and transcription factors such as AP2, Gbx2, Pax3 and Zic1. Among them, Pax3 and Zic1 are both necessary and sufficient to trigger a complete neural crest developmental program. However, their gene targets in the neural crest regulatory network remain unknown. Here, through a transcriptome analysis of frog microdissected neural border, we identified an extended gene signature for the premigratory neural crest, and we defined novel potential members of the regulatory network. This signature includes 34 novel genes, as well as 44 known genes expressed at the neural border. Using another microarray analysis which combined Pax3 and Zic1 gain-of-function and protein translation blockade, we uncovered 25 Pax3 and Zic1 direct targets within this signature. We demonstrated that the neural border specifiers Pax3 and Zic1 are direct upstream regulators of neural crest specifiers Snail1/2, Foxd3, Twist1, and Tfap2b. In addition, they may modulate the transcriptional output of multiple signaling pathways involved in neural crest development (Wnt, Retinoic Acid) through the induction of key pathway regulators (Axin2 and Cyp26c1). We also found that Pax3 could maintain its own expression through a positive autoregulatory feedback loop. These hierarchical inductions, feedback loops, and pathway modulations provide novel tools to understand the neural crest induction network.

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Species referenced: Xenopus laevis
Genes referenced: acod1lb acta1 angpt4 anos1 axin2 bnip3 btc c3 capn8.1 col18a1 cyp26c1 dact1 dynll1 elavl3 ets1 fgfr4 fkbp9 foxd3 fst fzd10 gbx2 gbx2.2 glipr2 greb1l guk1 hapln3 hoxa2 hsp90aa1.1 irf1 irx1 irx2 irx3 lmx1b mafb meis3 mfap2 mmp28 msx1 msx2 myo10.2 myo1d nipal2 nrp1 olig4 pax3 pcdh8l pdgfra peli1 pfkfb4 phyhdlb plekhn1 pnhd prtg pts rab11fip4l rara ror2 sdhb snai1 snai2 sox10 sox9 tcf7 tenm4 tfap2a tfap2b tpbg tuba1cl.3 twist1 wls wnt1 wnt11b wnt8a zfp36l2 zic1

???displayArticle.gses??? GSE53677: NCBI
GSE53678: NCBI
GSE53679: NCBI

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References [+] :
Alarcón, A dual requirement for Iroquois genes during Xenopus kidney development. 2008, Pubmed, Xenbase