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Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.
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We have microinjected a mAb specifically directed to phosphatidylinositol 4,5-bisphosphate (PIP2) into one blastomere of two-cell stage Xenopus laevis embryos. This antibody binds to endogenous PIP2 and reduces its rate of hydrolysis by phospholipase C. Antibody-injected blastomeres undergo partial or complete arrest of the cell cycle whereas the uninjected sister blastomeres divided normally. Since PIP2 hydrolysis normally produces diacylglycerol (DG) and inositol 1,4,5-triphosphate (Ins[1,4,5]P3), we attempted to measure changes in the levels of DG following stimulation of PIP2 hydrolysis in antibody-injected oocytes. The total amount of DG in antibody-injected oocytes was significantly reduced compared to that of water-injected ones following stimulation by either acetylcholine or progesterone indicating that the antibody does indeed suppress PIP2 hydrolysis. We also found that the PIP2 antibodies greatly reduced the amount of intracellular Ca2+ released in the egg cortex during egg activation. As an indirect test for Ins(1,4,5)P3 involvement in the cell cycle we injected heparin which competes with Ins(1,4,5)P3 for binding to its receptor, and thus inhibits Ins(1,4,5)P3-induced Ca2+ release. Microinjection of heparin into one blastomere of the two-cell stage embryo caused partial or complete arrest of the cell cycle depending upon the concentration of heparin injected. We further investigated the effect of reducing any [Ca2+]i gradients by microinjecting dibromo-BAPTA into the blastomere. Dibromo-BAPTA injection completely blocked mitotic cell division when a final concentration of 1.5 mM was used. These results suggest that PIP2 turnover as well as second messenger activity influence cell cycle duration during embryonic cell division in frogs.
Baker,
Intracellular calcium and cell cleavage in early embryos of Xenopus laevis.
1972, Pubmed,
Xenbase
Baker,
Intracellular calcium and cell cleavage in early embryos of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Baltus,
Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.
1988,
Pubmed
Berridge,
Inositol phosphates and cell signalling.
1989,
Pubmed
Broek,
The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway.
1987,
Pubmed
Cataldi,
Transient shift of diacylglycerol and inositol lipids induced by interferon in Daudi cells. Evidence for a different pattern between nuclei and intact cells.
1990,
Pubmed
Chafouleas,
Changes in calmodulin and its mRNA accompany reentry of quiescent (G0) cells into the cell cycle.
1984,
Pubmed
Chafouleas,
Calmodulin and the cell cycle: involvement in regulation of cell-cycle progression.
1982,
Pubmed
Chilvers,
Determination of mass changes in phosphatidylinositol 4,5-bisphosphate and evidence for agonist-stimulated metabolism of inositol 1,4,5-trisphosphate in airway smooth muscle.
1991,
Pubmed
Ciapa,
Two phases of inositol polyphosphate and diacylglycerol production at fertilisation.
1986,
Pubmed
Cocco,
Changes in nuclear inositol phospholipids induced in intact cells by insulin-like growth factor I.
1989,
Pubmed
Cocco,
Synthesis of polyphosphoinositides in nuclei of Friend cells. Evidence for polyphosphoinositide metabolism inside the nucleus which changes with cell differentiation.
1987,
Pubmed
Cocco,
Rapid changes in phospholipid metabolism in the nuclei of Swiss 3T3 cells induced by treatment of the cells with insulin-like growth factor I.
1988,
Pubmed
Dargie,
Comparison of Ca2+ mobilizing activities of cyclic ADP-ribose and inositol trisphosphate.
1990,
Pubmed
Deshpande,
Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.
1987,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Ferguson,
The effects of inositol trisphosphates and inositol tetrakisphosphate on Ca2+ release and Cl- current pattern in the Xenopus laevis oocyte.
1991,
Pubmed
,
Xenbase
Ferris,
Purified inositol 1,4,5-trisphosphate receptor mediates calcium flux in reconstituted lipid vesicles.
1989,
Pubmed
Fluck,
Slow calcium waves accompany cytokinesis in medaka fish eggs.
1991,
Pubmed
Forer,
The role of the phosphatidylinositol cycle in mitosis in sea urchin zygotes. Lithium inhibition is overcome by myo-inositol but not by other cyclitols or sugars.
1987,
Pubmed
Fukami,
Quantitative changes in polyphosphoinositides 1,2-diacylglycerol and inositol 1,4,5-trisphosphate by platelet-derived growth factor and prostaglandin F2 alpha.
1989,
Pubmed
Fukami,
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
1988,
Pubmed
Ghosh,
Competitive, reversible, and potent antagonism of inositol 1,4,5-trisphosphate-activated calcium release by heparin.
1988,
Pubmed
Gibbs,
Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
1984,
Pubmed
Grandin,
Intracellular free calcium oscillates during cell division of Xenopus embryos.
1991,
Pubmed
,
Xenbase
HIRAMOTO,
Microinjection of the live spermatozoa into sea urchin eggs.
1962,
Pubmed
Han,
Inositol 1,4,5-trisphosphate-induced calcium release in the organelle layers of the stratified, intact egg of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Hepler,
Calcium transients during mitosis: observations in flux.
1989,
Pubmed
Hepler,
Calcium restriction prolongs metaphase in dividing Tradescantia stamen hair cells.
1985,
Pubmed
Hill,
Heparin inhibits inositol trisphosphate-induced calcium release from permeabilized rat liver cells.
1987,
Pubmed
Hirono,
Neurotensin and acetylcholine evoke common responses in frog oocytes injected with rat brain messenger ribonucleic acid.
1987,
Pubmed
,
Xenbase
Huang,
Platelet-derived growth factor-mediated Ca2+ entry is blocked by antibodies to phosphatidylinositol 4,5-bisphosphate but does not involve heparin-sensitive inositol 1,4,5-trisphosphate receptors.
1991,
Pubmed
Hughes,
Homologous activators of ras in fission and budding yeast.
1990,
Pubmed
Izant,
The role of calcium ions during mitosis. Calcium participates in the anaphase trigger.
1983,
Pubmed
Kao,
Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts.
1990,
Pubmed
Keith,
Intracellular free calcium levels are reduced in mitotic Pt K2 epithelial cells.
1985,
Pubmed
Keith,
Local cytoplasmic calcium gradients in living mitotic cells.
,
Pubmed
Kiehart,
Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system.
1981,
Pubmed
Kline,
Evidence for the involvement of a pertussis toxin-insensitive G-protein in egg activation of the frog, Xenopus laevis.
1991,
Pubmed
,
Xenbase
Lacal,
Ras p21 proteins with high or low GTPase activity can efficiently transform NIH/3T3 cells.
1986,
Pubmed
Lacal,
Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21.
1987,
Pubmed
,
Xenbase
Levin,
A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle.
1990,
Pubmed
Matuoka,
Mitogenesis in response to PDGF and bombesin abolished by microinjection of antibody to PIP2.
1988,
Pubmed
Miron,
Cytological effects of the microinjection of antibody to ras p21 in early cleavage Xenopus embryos.
1990,
Pubmed
,
Xenbase
Murray,
Dominoes and clocks: the union of two views of the cell cycle.
1989,
Pubmed
Newmark,
Events at the surface of the cell.
,
Pubmed
Nishihara,
Inositol phospholipid levels of rat forebrain obtained by freeze-blowing method.
1985,
Pubmed
Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase
Picard,
Microinjection of a conserved peptide sequence of p34cdc2 induces a Ca2+ transient in oocytes.
1990,
Pubmed
,
Xenbase
Poenie,
Changes of free calcium levels with stages of the cell division cycle.
,
Pubmed
Poenie,
Calcium rises abruptly and briefly throughout the cell at the onset of anaphase.
1986,
Pubmed
Rakow,
Multiple stores of calcium are released in the sea urchin egg during fertilization.
1990,
Pubmed
Ratan,
Transition from metaphase to anaphase is accompanied by local changes in cytoplasmic free calcium in Pt K2 kidney epithelial cells.
1986,
Pubmed
Rink,
Free calcium in Xenopus embryos measured with ion-selective microelectrodes.
1980,
Pubmed
,
Xenbase
Robinson,
CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae.
1987,
Pubmed
Shih,
Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus.
1980,
Pubmed
Silver,
Nuclear envelope breakdown and mitosis in sand dollar embryos is inhibited by microinjection of calcium buffers in a calcium-reversible fashion, and by antagonists of intracellular Ca2+ channels.
1989,
Pubmed
Speksnijder,
Calcium buffer injections block fucoid egg development by facilitating calcium diffusion.
1989,
Pubmed
Steinhardt,
Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo.
1988,
Pubmed
Tsien,
New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures.
1980,
Pubmed
Twigg,
Translational control of InsP3-induced chromatin condensation during the early cell cycles of sea urchin embryos.
1988,
Pubmed
Uno,
Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation.
1988,
Pubmed
Wasserman,
sn-1,2-diacylglycerol levels increase in progesterone-stimulated Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Whitaker,
Calcium and cell cycle control.
1990,
Pubmed
Worley,
Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.
1987,
Pubmed
Zhang,
Regulation of anaphase chromosome motion in Tradescantia stamen hair cells by calcium and related signaling agents.
1990,
Pubmed
