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Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes.
Schmalzing G
,
Kröner S
,
Passow H
.
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Ouabain binding was studied in Xenopus laevis oocytes permeabilized by detergents. The behaviour of markers showed that 10 microM-digitonin selectively disrupts the plasma membrane. In the presence of ATP, oocytes permeabilized at 10 microM-digitonin bound no more ouabain molecules than were required to abolish active 86Rb+ uptake in the intact cells. However, the ouabain binding capacity increased approx. 2-fold when inner membranes were disrupted by SDS or excess digitonin, as judged from the accompanying release of the lysosomal marker beta-hexosaminidase. The results suggest that oocytes have a large internal pool of functional sodium pumps.
Caplan,
Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells.
1986, Pubmed
Caplan,
Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells.
1986,
Pubmed
Churchill,
Biosynthesis and insertion of (Na+ + K+)-adenosine triphosphatase subunits into eel electroplax membranes.
1979,
Pubmed
Cook,
Uptake of [3H]ouabain from the cell surface into the lysosomal compartment of HeLa cells.
1982,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Geering,
Maturation of the catalytic alpha-subunit of Na,K-ATPase during intracellular transport.
1987,
Pubmed
,
Xenbase
Geering,
Membrane insertion of alpha- and beta-subunits of Na+,K+-ATPase.
1985,
Pubmed
,
Xenbase
Gögelein,
Interaction of saponin and digitonin with black lipid membranes and lipid monolayers.
1984,
Pubmed
Kashgarian,
Monoclonal antibody to Na,K-ATPase: immunocytochemical localization along nephron segments.
1985,
Pubmed
Mircheff,
Basal-lateral and intracellular membrane populations of rat exorbital lacrimal gland.
1983,
Pubmed
Morgan,
Mediation of anion transport in oocytes of Xenopus laevis by biosynthetically inserted band 3 protein from mouse spleen erythroid cells.
1985,
Pubmed
,
Xenbase
Richter,
Regulatory changes of membrane transport and ouabain binding during progesterone-induced maturation of Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Tamkun,
The (Na+ + K+)-ATPase of chick sensory neurons. Studies on biosynthesis and intracellular transport.
1986,
Pubmed
Wall,
An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.
1985,
Pubmed
,
Xenbase